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PMID: 4018036 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel virulence-associated cell surface structure composed of 47-kd protein subunits in Yersinia enterocolitica.

The EMBO journal ·Vol. 4 ·No. 4 ·1985-04-00 ·Pages 1013-8

Zaleska M, Lounatmaa K, Nurminen M, Wahlström E, Mäkelä PH

Abstract

A fresh human isolate of Yersinia enterocolitica serotype 03, and its derivative that had lost the virulence-associated 46-Md plasmid, were grown under defined conditions and compared for their outer membrane protein and cell surface structure. Under these conditions, the virulent strain grown at 37 degrees C expressed one major outer membrane protein (47 kd) not present in the plasmidless strain or in either strain grown at room temperature. A 200-kd protein also seen in the same preparations was shown to be an oligomer composed of the 47-kd protein subunits. Four different electron microscopic techniques showed tack-like projections covering the surface of those bacteria that expressed the 47-kd protein. These were specifically labeled with antibody to the 47-kd protein. This surface structure appeared to mediate aggregation (auto-agglutination) of the bacteria bringing their surfaces into unusually close apposition.

MeSH Terms
Bacterial Outer Membrane Proteins/analysis Electrophoresis, Polyacrylamide Gel Humans Macromolecular Substances Microscopy, Electron Molecular Weight Yersinia/isolation & purification,ultrastructure Yersinia enterocolitica/pathogenicity
Chemicals
Bacterial Outer Membrane Proteins Macromolecular Substances
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zaleska M
Lounatmaa K
Nurminen M
Wahlström E
Mäkelä P H
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-04-00
Pages
1013-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554293
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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