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PMID: 7275932 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and disposition of a surface protein associated with virulence of Aeromonas salmonicida.

Journal of bacteriology ·Vol. 147 ·No. 3 ·1981-09-00 ·Pages 1077-84

Kay WW, Buckley JT, Ishiguro EE, Phipps BM, Monette JP, Trust TJ

Abstract

Virulent strains of Aeromonas salmonicida observed by electron microscopy were characterized by an outer layer exhibiting a tetragonal repeat pattern. Attenuated strains had a 2.5 X 10(3)- to 5 X 10(3)-fold reduction in virulence and lost the outer layer, autoaggregating properties, and a 49-kilodalton protein (A protein) simultaneously. The A protein is the major protein component of outer membrane fractions of virulent strains. A variety of radiolabeling studies showed that this protein was surface localized and that it provided an effective barrier against iodination of other outer membrane proteins with either lactoperoxidase or diazoiodosulfanilic acid; A protein was not labeled with lactoperoxidase but was specifically labeled with diazoidosulfanilic acid. The A protein was purified by selective extraction with detergent and guanidine hydrochloride, and its amino acid composition was determined. The properties of A protein are compared with those of other bacterial surface layer proteins.

MeSH Terms

Aeromonas/analysis,pathogenicity Amino Acids/analysis Bacterial Proteins/analysis,isolation & purification Membrane Proteins/analysis,isolation & purification

Chemicals

Amino Acids Bacterial Proteins Membrane Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kay W W

Buckley J T

Ishiguro E E

Phipps B M

Monette J P

Trust T J

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23 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.147.3.1077-1084.1981

Published

1981-09-00

Pages

1077-84

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC216148

Subset

External Links

PubMed source DOI: 10.1128/jb.147.3.1077-1084.1981 PMC Full Text View journal details

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Purification and disposition of a surface protein associated with virulence of Aeromonas salmonicida.

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