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PMID: 11101532 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast.

The EMBO journal ·Vol. 19 ·No. 23 ·2000-12-01 ·Pages 6602-11

Olivas W, Parker R

Abstract

Eukaryotic post-transcriptional regulation is often specified by control elements within mRNA 3'- untranslated regions (3'-UTRs). In order to identify proteins that regulate specific mRNA decay rates in Saccharomyces cerevisae, we analyzed the role of five members of the Puf family present in the yeast genome (referred to as JSN1/PUF1, PUF2, PUF3, PUF4 and MPT5/PUF5). Yeast strains lacking all five Puf proteins showed differential expression of numerous yeast mRNAs. Examination of COX17 mRNA indicates that Puf3p specifically promotes decay of this mRNA by enhancing the rate of deadenylation and subsequent turnover. Puf3p also binds to the COX17 mRNA 3'-UTR in vitro. This indicates that the function of Puf proteins as specific regulators of mRNA deadenylation has been conserved throughout eukaryotes. In contrast to the case in Caenorhabditis elegans and Drosophila, yeast Puf3p does not affect translation of COX17 mRNA. These observations indicate that Puf proteins are likely to play a role in the control of transcript-specific rates of degradation in yeast by interacting directly with the mRNA turnover machinery.

MeSH Terms
3' Untranslated Regions Alleles Animals Base Sequence Blotting, Northern Blotting, Western Caenorhabditis elegans/metabolism Cation Transport Proteins Copper Transport Proteins Drosophila Models, Genetic Molecular Chaperones Molecular Sequence Data Multigene Family Mutagenesis Oligonucleotide Array Sequence Analysis Open Reading Frames Poly A Polyribosomes/metabolism Protein Binding Protein Biosynthesis Proteins/metabolism RNA Processing, Post-Transcriptional RNA, Messenger/metabolism RNA-Binding Proteins/physiology Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Time Factors Transcription, Genetic Yeasts/metabolism
Chemicals
3' Untranslated Regions COX17 protein, S cerevisiae Cation Transport Proteins Copper Transport Proteins Molecular Chaperones Proteins RNA, Messenger RNA-Binding Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Poly A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Olivas W
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA.
Parker R
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.23.6602
Published
2000-12-01
Pages
6602-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305854
Subset
IM
Grants
NIGMS NIH HHS · GM4544 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.23.6602 PMC Full Text View journal details
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