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PMID: 9154842 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest.

Molecular and cellular biology ·Vol. 17 ·No. 6 ·1997-06-00 ·Pages 3429-39

Chen T, Kurjan J

Abstract

SST2 plays an important role in the sensitivity of yeast cells to pheromone and in recovery from pheromone-induced G1 arrest. Recently, a family of Sst2p homologs that act as GTPase-activating proteins (GAPs) for G alpha subunits has been identified. We have identified an interaction between Sst2p and the previously identified Mpt5p by using the two-hybrid system. Loss of Mpt5p function resulted in a temperature-sensitive growth phenotype, an increase in pheromone sensitivity, and a defect in recovery from pheromone-induced G1 arrest, although the effects on pheromone response and recovery were mild in comparison to those of sst2 mutants. Overexpression of either Sst2p or Mpt5p promoted recovery from G1 arrest. Promotion of recovery by overexpression of Mpt5p required Sst2p, but the effect of overexpression of Sst2p was only partially dependent on Mpt5p. Mpt5p was also found to interact with the mitogen-activated protein kinase homologs Fus3p and Kss1p, and an mpt5 mutation was able to suppress the pheromone arrest and mating defects of a fus3 mutant. Because either mpt5 or cln3 mutations suppressed the fus3 phenotypes, interactions of Mpt5p with the G1 cyclins and Cdc28p were tested. An interaction between Mpt5p and Cdc28p was detected. We discuss these results with respect to a model in which Sst2p plays a role in pheromone sensitivity and recovery that acts through Mpt5p in addition to a role as a G alpha GAP suggested by the analysis of the Sst2p homologs.

MeSH Terms
CDC28 Protein Kinase, S cerevisiae/metabolism Calcium-Calmodulin-Dependent Protein Kinases/metabolism Carrier Proteins/metabolism Cell Cycle Fungal Proteins/metabolism G1 Phase GTPase-Activating Proteins Mitogen-Activated Protein Kinases Models, Chemical Phenotype Pheromones/physiology Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Chemicals
Carrier Proteins Fungal Proteins GTPase-Activating Proteins Pheromones SST2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Calcium-Calmodulin-Dependent Protein Kinases CDC28 Protein Kinase, S cerevisiae FUS3 protein, S cerevisiae Mitogen-Activated Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen T
Department of Microbiology and Molecular Genetics, College of Medicine, University of Vermont, Burlington 05405-0068, USA.
Kurjan J
References (71)
71 references, click to expand
  1. FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1.
    Mol Biol Cell. 1993 May;4(5):495-510 PMID: 8334305
  2. A new yeast gene, HTR1, required for growth at high temperature, is needed for recovery from mating pheromone-induced G1 arrest.
    Mol Gen Genet. 1994 Oct 17;245(1):107-16 PMID: 7845352
  3. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade.
    Genetics. 1994 Nov;138(3):609-19 PMID: 7851759
  4. Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos.
    Cell. 1995 Mar 10;80(5):747-56 PMID: 7889568
  5. Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae.
    Mol Cell Biol. 1995 Jul;15(7):3635-43 PMID: 7791771
  6. The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene.
    Yeast. 1995 Jun 30;11(8):767-74 PMID: 7668046
  7. GAIP, a protein that specifically interacts with the trimeric G protein G alpha i3, is a member of a protein family with a highly conserved core domain.
    Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11916-20 PMID: 8524874
  8. There are GAPS and there are GAPS.
    Science. 1997 Jan 3;275(5296):42-3 PMID: 8999536
  9. The proliferation of MAP kinase signaling pathways in yeast.
    Curr Opin Cell Biol. 1995 Apr;7(2):197-202 PMID: 7612271
  10. Genetic relationships between the G protein beta gamma complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway.
    Genetics. 1996 May;143(1):103-17 PMID: 8722766
  11. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit).
    Mol Cell Biol. 1996 Sep;16(9):5194-209 PMID: 8756677
  12. The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development.
    EMBO J. 1996 Oct 1;15(19):5184-90 PMID: 8895563
  13. Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities.
    Mol Cell Biol. 1996 Dec;16(12):6794-803 PMID: 8943334
  14. RGS4 and GAIP are GTPase-activating proteins for Gq alpha and block activation of phospholipase C beta by gamma-thio-GTP-Gq alpha.
    Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):428-32 PMID: 9012799
  15. RGS proteins and signaling by heterotrimeric G proteins.
    J Biol Chem. 1997 Feb 14;272(7):3871-4 PMID: 9064301
  16. Far1 and Fus3 link the mating pheromone signal transduction pathway to three G1-phase Cdc28 kinase complexes.
    Mol Cell Biol. 1993 Sep;13(9):5659-69 PMID: 8395009
  17. Glucose uptake and catabolite repression in dominant HTR1 mutants of Saccharomyces cerevisiae.
    J Bacteriol. 1993 Sep;175(17):5520-8 PMID: 8366037
  18. Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast.
    Yeast. 1993 Jul;9(7):715-22 PMID: 8368005
  19. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
    Cell. 1993 Nov 19;75(4):805-16 PMID: 8242751
  20. The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.
    Genes Dev. 1994 Feb 1;8(3):313-27 PMID: 8314085
  21. The pheromone response pathway in Saccharomyces cerevisiae.
    Annu Rev Genet. 1993;27:147-79 PMID: 8122900
  22. A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog.
    Mol Biol Cell. 1994 Mar;5(3):297-312 PMID: 8049522
  23. Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module.
    Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7762-6 PMID: 8052657
  24. Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae.
    Cell. 1994 Aug 12;78(3):499-512 PMID: 8062390
  25. Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1.
    Science. 1994 Aug 26;265(5176):1228-31 PMID: 8066461
  26. Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development.
    Mol Microbiol. 1994 Oct;14(2):323-34 PMID: 7830576
  27. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):11-20 PMID: 7050665
  28. Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):21-9 PMID: 7050666
  29. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  30. Comparison of dose-response curves for alpha factor-induced cell division arrest, agglutination, and projection formation of yeast cells. Implication for the mechanism of alpha factor action.
    J Biol Chem. 1983 Nov 25;258(22):13849-56 PMID: 6358212
  31. Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.
    DNA. 1984 Dec;3(6):479-88 PMID: 6096101
  32. Nucleotide sequence of a yeast Ty element: evidence for an unusual mechanism of gene expression.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2829-33 PMID: 2581255
  33. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):735-9 PMID: 3003748
  34. Regulation of the yeast HO gene.
    Cold Spring Harb Symp Quant Biol. 1985;50:643-50 PMID: 3938367
  35. Chemical synthesis and expression of a cassette adapted ubiquitin gene.
    J Biol Chem. 1987 Mar 15;262(8):3524-7 PMID: 3029116
  36. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
    Mol Cell Biol. 1987 Jul;7(7):2316-28 PMID: 3302672
  37. The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway.
    Cell. 1987 Sep 25;50(7):1001-10 PMID: 3113738
  38. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction.
    Cell. 1987 Sep 25;50(7):1011-9 PMID: 3113739
  39. Pheromonal regulation and sequence of the Saccharomyces cerevisiae SST2 gene: a model for desensitization to pheromone.
    Mol Cell Biol. 1987 Dec;7(12):4169-77 PMID: 2830483
  40. DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Nov;8(11):4675-84 PMID: 3062366
  41. The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein.
    Cell. 1989 Feb 10;56(3):467-77 PMID: 2536595
  42. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  43. The yeast STE12 protein binds to the DNA sequence mediating pheromone induction.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5703-7 PMID: 2668945
  44. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae.
    Cell. 1989 Sep 22;58(6):1107-19 PMID: 2673544
  45. An essential G1 function for cyclin-like proteins in yeast.
    Cell. 1989 Dec 22;59(6):1127-33 PMID: 2574633
  46. STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes.
    Genes Dev. 1989 Sep;3(9):1349-61 PMID: 2558054
  47. FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
    Cell. 1990 Feb 23;60(4):649-64 PMID: 2406028
  48. Effects of expression of mammalian G alpha and hybrid mammalian-yeast G alpha proteins on the yeast pheromone response signal transduction pathway.
    Mol Cell Biol. 1990 Jun;10(6):2582-90 PMID: 2111439
  49. Cloning and nucleotide sequence of the CDC23 gene of Saccharomyces cerevisiae.
    Gene. 1990 Jul 2;91(1):123-6 PMID: 2205535
  50. Cell cycle arrest caused by CLN gene deficiency in Saccharomyces cerevisiae resembles START-I arrest and is independent of the mating-pheromone signalling pathway.
    Mol Cell Biol. 1990 Dec;10(12):6482-90 PMID: 2147225
  51. Putting the HO gene to work: practical uses for mating-type switching.
    Methods Enzymol. 1991;194:132-46 PMID: 2005783
  52. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.
    Genes Dev. 1991 May;5(5):741-50 PMID: 2026326
  53. Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase.
    J Biol Chem. 1991 Aug 15;266(23):15180-4 PMID: 1907971
  54. The YGL023 gene encodes a putative regulatory protein.
    Yeast. 1991 Apr;7(3):309-12 PMID: 1909073
  55. FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9392-6 PMID: 1946350
  56. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9578-82 PMID: 1946372
  57. Multifunctional yeast high-copy-number shuttle vectors.
    Gene. 1992 Jan 2;110(1):119-22 PMID: 1544568
  58. Signal transduction during pheromone response in yeast.
    Annu Rev Cell Biol. 1991;7:699-728 PMID: 1667085
  59. The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein.
    Development. 1992 Jan;114(1):221-32 PMID: 1576962
  60. A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation.
    Genes Dev. 1992 May;6(5):801-14 PMID: 1577274
  61. The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation.
    EMBO J. 1992 May;11(5):1773-84 PMID: 1316273
  62. Mitogen-activated protein kinases: versatile transducers for cell signaling.
    Trends Biochem Sci. 1992 Jun;17(6):233-8 PMID: 1323888
  63. Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos.
    Genes Dev. 1992 Dec;6(12A):2312-26 PMID: 1459455
  64. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components.
    EMBO J. 1992 Dec;11(13):4815-24 PMID: 1464311
  65. Molecular analysis of POP2 gene, a gene required for glucose-derepression of gene expression in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1992 Dec 11;20(23):6227-33 PMID: 1475183
  66. A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):452-6 PMID: 8421676
  67. MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro.
    Nature. 1993 Mar 18;362(6417):261-4 PMID: 8384702
  68. Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins.
    EMBO J. 1993 May;12(5):1955-68 PMID: 8387915
  69. FAR1 links the signal transduction pathway to the cell cycle machinery in yeast.
    Cell. 1993 May 21;73(4):747-60 PMID: 8500168
  70. Targeting of a heterologous protein to the cell wall of Saccharomyces cerevisiae.
    Yeast. 1993 Apr;9(4):399-409 PMID: 8390128
  71. Elimination of false positives that arise in using the two-hybrid system.
    Biotechniques. 1993 Jun;14(6):920-4 PMID: 8333960
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.6.3429
Published
1997-06-00
Pages
3429-39
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232196
Subset
IM
Grants
NIGMS NIH HHS · GM40585 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.6.3429 PMC Full Text View journal details
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