Home LiteratureArticle Details
PMID: 3062366 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4675-84

Cross FR

Abstract

The mating pheromone alpha-factor arrests Saccharomyces cerevisiae MATa cells in the G1 phase of the cell cycle. Size control is also exerted in G1, since cells do not exit G1 until they have attained a critical size. A dominant mutation (DAF1-1) which causes both alpha-factor resistance and small cell size (volume about 0.6-fold that of the wild type) has been isolated and characterized genetically and by molecular cloning. Several alpha-factor-induced mRNAs were induced equivalently in daf1+ and DAF1-1 cells. The DAF1-1 mutation consisted of a termination codon two-thirds of the way through the daf1+ coding sequence. A chromosomal deletion of DAF1 produced by gene transplacement increased cell volume about 1.5-fold; thus, DAF1-1 may be a hyperactive or deregulated allele of a nonessential gene involved in G1 size control. Multiple copies of DAF1-1 also greatly reduced the duration of the G1 phase of the cell cycle.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle DNA, Fungal/biosynthesis,genetics Genes, Fungal Kinetics Molecular Sequence Data Mutation Phenotype Pheromones/pharmacology Plasmids Restriction Mapping Saccharomyces cerevisiae/cytology,drug effects,genetics
Chemicals
DNA, Fungal Pheromones
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cross F R
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
References (63)
63 references, click to expand
  1. Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog.
    Cell. 1987 May 22;49(4):559-67 PMID: 3032459
  2. Dual regulation of the yeast CDC28-p40 protein kinase complex: cell cycle, pheromone, and nutrient limitation effects.
    Cell. 1987 Sep 11;50(6):927-35 PMID: 3040265
  3. Functionally homologous cell cycle control genes in budding and fission yeast.
    Nature. 1982 Dec 23;300(5894):706-9 PMID: 6757758
  4. Comparison of dose-response curves for alpha factor-induced cell division arrest, agglutination, and projection formation of yeast cells. Implication for the mechanism of alpha factor action.
    J Biol Chem. 1983 Nov 25;258(22):13849-56 PMID: 6358212
  5. Small-sized mutants of Saccharomyces cerevisiae.
    Genetics. 1980 Nov;96(3):561-6 PMID: 7021310
  6. Yeast STE7, STE11, and STE12 genes are required for expression of cell-type-specific genes.
    Mol Cell Biol. 1988 Feb;8(2):551-6 PMID: 3280969
  7. Induction of yeast mating pheromone a-factor by alpha cells.
    Nature. 1983 Oct 6-12;305(5934):543-5 PMID: 6353246
  8. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
    Mol Cell Biol. 1987 Jul;7(7):2316-28 PMID: 3302672
  9. Yeast cells recover from mating pheromone alpha factor-induced division arrest by desensitization in the absence of alpha factor destruction.
    J Biol Chem. 1984 Jan 25;259(2):1004-10 PMID: 6363399
  10. Control of timing of cell cycle events in fission yeast by the wee 1+ gene.
    Nature. 1983 Mar 10;302(5904):153-5 PMID: 6828164
  11. Molecular cloning of hormone-responsive genes from the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):1144-8 PMID: 6366798
  12. Control of cell size at division in fission yeast by a growth-modulated size control over nuclear division.
    Exp Cell Res. 1977 Jul;107(2):377-86 PMID: 872891
  13. Size control models of Saccharomyces cerevisiae cell proliferation.
    Mol Cell Biol. 1982 Apr;2(4):361-8 PMID: 7050671
  14. Mutants altered in the control co-ordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe.
    Mol Gen Genet. 1978 May 3;161(2):215-20 PMID: 672898
  15. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants.
    Genetics. 1974 Feb;76(2):255-71 PMID: 4595643
  16. Genetic map of Saccharomyces cerevisiae, edition 9.
    Microbiol Rev. 1985 Sep;49(3):181-213 PMID: 2995780
  17. Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae.
    EMBO J. 1985 Oct;4(10):2643-8 PMID: 16453635
  18. Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins.
    Mol Cell Biol. 1986 Nov;6(11):3722-33 PMID: 3540600
  19. The yeast STE12 product is required for expression of two sets of cell-type specific genes.
    Cell. 1985 Oct;42(3):923-30 PMID: 3931921
  20. The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway.
    Cell. 1987 Sep 25;50(7):1001-10 PMID: 3113738
  21. Yeast promoters: positive and negative elements.
    Cell. 1984 Apr;36(4):799-800 PMID: 6368004
  22. Down regulation of the alpha-factor pheromone receptor in S. cerevisiae.
    Cell. 1986 Aug 1;46(3):345-53 PMID: 3015412
  23. Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8419-23 PMID: 3909145
  24. A rapid, efficient method for isolating DNA from yeast.
    Gene. 1986;42(2):169-73 PMID: 3015730
  25. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus.
    Cell. 1982 Nov;31(1):183-92 PMID: 6297747
  26. Induction of the yeast alpha-specific STE3 gene by the peptide pheromone a-factor.
    J Mol Biol. 1984 Oct 5;178(4):835-52 PMID: 6436496
  27. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and characterization of the CDC24 gene and adjacent regions of the chromosome.
    Mol Cell Biol. 1986 Dec;6(12):4516-25 PMID: 3540615
  28. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.
    J Cell Biol. 1980 Jun;85(3):811-22 PMID: 6993497
  29. Yeast peptide pheromones, a-factor and alpha-factor, activate a common response mechanism in their target cells.
    Cell. 1986 Dec 26;47(6):929-37 PMID: 3022943
  30. A control acting over the initiation of DNA replication in the yeast Schizosaccharomyces pombe.
    J Cell Sci. 1979 Apr;36:155-68 PMID: 457803
  31. Fine structure recombinational analysis of cloned genes using yeast transformation.
    Genetics. 1987 Jan;115(1):73-81 PMID: 3549444
  32. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae.
    Exp Cell Res. 1977 Mar 1;105(1):79-98 PMID: 320023
  33. Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division.
    J Cell Biol. 1977 Nov;75(2 Pt 1):422-35 PMID: 400873
  34. Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression.
    Mol Cell Biol. 1987 Apr;7(4):1311-9 PMID: 3037311
  35. Controls over the timing of DNA replication during the cell cycle of fission yeast.
    Exp Cell Res. 1977 Jul;107(2):365-75 PMID: 872890
  36. Genetic and biochemical characterization of clathrin-deficient Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Nov;7(11):3888-98 PMID: 3323882
  37. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  38. Binding of alpha-factor pheromone to Saccharomyces cerevisiae a cells: dissociation constant and number of binding sites.
    Mol Cell Biol. 1986 Jan;6(1):318-20 PMID: 3023832
  39. Expression of the BAR1 gene in Saccharomyces cerevisiae: induction by the alpha mating pheromone of an activity associated with a secreted protein.
    J Bacteriol. 1983 Jul;155(1):291-301 PMID: 6345506
  40. The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.
    Cell. 1987 May 22;49(4):569-76 PMID: 3453113
  41. Genetic control of cell division in yeast cultured at different growth rates.
    Nature. 1977 Sep 8;269(5624):145-7 PMID: 333292
  42. Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Aug;7(8):2680-90 PMID: 3313002
  43. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):11-20 PMID: 7050665
  44. Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast.
    Nature. 1981 Aug 6;292(5823):558-60 PMID: 7254352
  45. ran1+ controls the transition from mitotic division to meiosis in fission yeast.
    Curr Genet. 1985;10(4):297-311 PMID: 3870979
  46. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor.
    Exp Cell Res. 1973 Jan;76(1):99-110 PMID: 4566314
  47. Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor.
    Cell. 1983 Dec;35(2 Pt 1):521-9 PMID: 6360378
  48. Multiple regulation of STE2, a mating-type-specific gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jun;6(6):2106-14 PMID: 3023919
  49. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  50. The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.
    Cell. 1986 Apr 25;45(2):261-8 PMID: 3516412
  51. Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling.
    Mol Cell Biol. 1988 Jun;8(6):2484-93 PMID: 3136318
  52. Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae.
    Yeast. 1985 Dec;1(2):83-138 PMID: 3916863
  53. Genes which control cell proliferation in the yeast Saccharomyces cerevisiae.
    Nature. 1980 Nov 27;288(5789):401-4 PMID: 7001255
  54. Genetic applications of yeast transformation with linear and gapped plasmids.
    Methods Enzymol. 1983;101:228-45 PMID: 6310326
  55. Control of cell division in Saccharomyces cerevisiae mutants defective in adenylate cyclase and cAMP-dependent protein kinase.
    Exp Cell Res. 1983 Jun;146(1):151-61 PMID: 6305691
  56. Unidirectional digestion with exonuclease III in DNA sequence analysis.
    Methods Enzymol. 1987;155:156-65 PMID: 3323819
  57. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction.
    Cell. 1987 Sep 25;50(7):1011-9 PMID: 3113739
  58. The selection of S. cerevisiae mutants defective in the start event of cell division.
    Genetics. 1980 Jul;95(3):561-77 PMID: 7002718
  59. A yeast operator overlaps an upstream activation site.
    Cell. 1987 Jul 31;50(3):369-77 PMID: 3301002
  60. The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
    J Biol Chem. 1982 Mar 25;257(6):3018-25 PMID: 6277922
  61. Transcriptional regulation in the yeast life cycle.
    Science. 1987 Sep 4;237(4819):1162-70 PMID: 3306917
  62. Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1418-22 PMID: 3006051
  63. The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene.
    Nucleic Acids Res. 1985 Dec 9;13(23):8463-75 PMID: 3001640
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.11.4675-4684.1988
Published
1988-11-00
Pages
4675-84
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365557
Subset
IM
Grants
NIGMS NIH HHS · GM26176 · United States
Databases
GENBANK
M23359
External Links
PubMed source DOI: 10.1128/mcb.8.11.4675-4684.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2