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PMID: 11102517 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mammalian meiotic telomeres: protein composition and redistribution in relation to nuclear pores.

Molecular biology of the cell ·Vol. 11 ·No. 12 ·2000-12-00 ·Pages 4189-203

Scherthan H, Jerratsch M, Li B, Smith S, Hultén M, Lock T, de Lange T

Abstract

Mammalian telomeres consist of TTAGGG repeats, telomeric repeat binding factor (TRF), and other proteins, resulting in a protective structure at chromosome ends. Although structure and function of the somatic telomeric complex has been elucidated in some detail, the protein composition of mammalian meiotic telomeres is undetermined. Here we show, by indirect immunofluorescence (IF), that the meiotic telomere complex is similar to its somatic counterpart and contains significant amounts of TRF1, TRF2, and hRap1, while tankyrase, a poly-(ADP-ribose)polymerase at somatic telomeres and nuclear pores, forms small signals at ends of human meiotic chromosome cores. Analysis of rodent spermatocytes reveals Trf1 at mouse, TRF2 at rat, and mammalian Rap1 at meiotic telomeres of both rodents. Moreover, we demonstrate that telomere repositioning during meiotic prophase occurs in sectors of the nuclear envelope that are distinct from nuclear pore-dense areas. The latter form during preleptotene/leptotene and are present during entire prophase I.

MeSH Terms
Animals Chromosomes/ultrastructure DNA-Binding Proteins/immunology,metabolism Fluorescent Antibody Technique, Indirect Humans Male Meiosis Mice Nuclear Pore/metabolism Poly(ADP-ribose) Polymerases/immunology,metabolism Prophase Rats Shelterin Complex Spermatocytes/cytology,metabolism Tankyrases Telomere/chemistry,metabolism Telomere-Binding Proteins Telomeric Repeat Binding Protein 1 Telomeric Repeat Binding Protein 2 rap1 GTP-Binding Proteins/immunology,metabolism
Chemicals
DNA-Binding Proteins Shelterin Complex TERF2IP protein, human Telomere-Binding Proteins Telomeric Repeat Binding Protein 1 Telomeric Repeat Binding Protein 2 Poly(ADP-ribose) Polymerases Tankyrases Tnks protein, mouse TNKS protein, human rap1 GTP-Binding Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Scherthan H
University of Kaiserslautern, D-67653 Kaiserslautern, Germany. [email protected]
Jerratsch M
Li B
Smith S
Hultén M
Lock T
de Lange T
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.12.4189
Email
[email protected]
Published
2000-12-00
Pages
4189-203
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC15066
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.11.12.4189 PMC Full Text View journal details
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