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PMID: 11018056 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Meiotic telomere protein Ndj1p is required for meiosis-specific telomere distribution, bouquet formation and efficient homologue pairing.

The Journal of cell biology ·Vol. 151 ·No. 1 ·2000-10-02 ·Pages 95-106

Trelles-Sticken E, Dresser ME, Scherthan H

Abstract

We have investigated the requirements for NDJ1 in meiotic telomere redistribution and clustering in synchronized cultures of Saccharomyces cerevisiae. On induction of wild-type meiosis, telomeres disperse from premeiotic aggregates over the nuclear periphery, and then cluster near the spindle pole body (bouquet arrangement) before dispersing again. In ndj1Delta meiocytes, telomeres are scattered throughout the nucleus and fail to form perinuclear meiosis-specific distribution patterns, suggesting that Ndj1p may function to tether meiotic telomeres to the nuclear periphery. Since ndj1Delta meiocytes fail to cluster their telomeres at any prophase stage, Ndj1p is the first protein shown to be required for bouquet formation in a synaptic organism. Analysis of homologue pairing by two-color fluorescence in situ hybridization with cosmid probes to regions on III, IX, and XI revealed that disruption of bouquet formation is associated with a significant delay (>2 h) of homologue pairing. An increased and persistent fraction of ndj1Delta meiocytes with Zip1p polycomplexes suggests that chromosome polarization is important for synapsis progression. Thus, our observations support the hypothesis that meiotic telomere clustering contributes to efficient homologue alignment and synaptic pairing. Under naturally occurring conditions, bouquet formation may allow for rapid sporulation and confer a selective advantage.

MeSH Terms
Centromere Chromosomal Proteins, Non-Histone/genetics Chromosome Painting Chromosomes, Fungal Gene Deletion Meiosis Models, Genetic Nuclear Proteins/genetics Saccharomyces cerevisiae/genetics Spindle Apparatus Telomere
Chemicals
Chromosomal Proteins, Non-Histone Nuclear Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Trelles-Sticken E
Department of Human Biology and Genetics, University of Kaiserslautern, D-67653 Kaiserslautern, Germany.
Dresser M E
Scherthan H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.151.1.95
Published
2000-10-02
Pages
95-106
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2189801
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.151.1.95 PMC Full Text View journal details
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