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PMID: 9335591 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway.

Genetics ·Vol. 147 ·No. 2 ·1997-10-00 ·Pages 533-44

Dresser ME, Ewing DJ, Conrad MN, Dominguez AM, Barstead R, Jiang H, Kodadek T

Abstract

Meiotic recombination in the yeast Saccharomyces cerevisiae requires two similar recA-like proteins, Dmc1p and Rad51p. A screen for dominant meiotic mutants provided DMC1-G126D, a dominant allele mutated in the conserved ATP-binding site (specifically, the A-loop motif) that confers a null phenotype. A recessive null allele, dmc1-K69E, was isolated as an intragenic suppressor of DMC1-G126D. Dmc1-K69Ep, unlike Dmc1p, does not interact homotypically in a two-hybrid assay, although it does interact with other fusion proteins identified by two-hybrid screen with Dmc1p. Dmc1p, unlike Rad51p, does not interact in the two-hybrid assay with Rad52p or Rad54p. However, Dmc1p does interact with Tid1p, a Rad54p homologue, with Tid4p, a Rad16p homologue, and with other fusion proteins that do not interact with Rad51p, suggesting that Dmc1p and Rad51p function in separate, though possibly overlapping, recombinational repair complexes. Epistasis analysis suggests that DMC1 and RAD51 function in separate pathways responsible for meiotic recombination. Taken together, our results are consistent with a requirement for DMC1 for meiosis-specific entry of DNA double-strand break ends into chromatin. Interestingly, the pattern on CHEF gels of chromosome fragments that result from meiotic DNA double-strand break formation is different in DMC1 mutant strains from that seen in rad50S strains.

MeSH Terms
Cell Cycle Proteins DNA Fragmentation DNA Repair DNA-Binding Proteins/genetics,metabolism Genes, Dominant Genes, Recessive Immunohistochemistry Meiosis/genetics Phenotype Protein Binding Rad51 Recombinase Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Cell Cycle Proteins DMC1 protein, S cerevisiae DNA-Binding Proteins Saccharomyces cerevisiae Proteins RAD51 protein, S cerevisiae Rad51 Recombinase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dresser M E
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA. [email protected]
Ewing D J
Conrad M N
Dominguez A M
Barstead R
Jiang H
Kodadek T
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
Published
1997-10-00
Pages
533-44
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1208176
Subset
IM
Grants
NIGMS NIH HHS · GM-45250-04 · United States
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