Home LiteratureArticle Details
PMID: 10385630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Somatic pairing of homologs in budding yeast: existence and modulation.

Genes & development ·Vol. 13 ·No. 12 ·1999-06-15 ·Pages 1627-41

Burgess SM, Kleckner N, Weiner BM

Abstract

FISH analysis of well-spread chromosomes reveals that homologs are paired in vegetatively growing budding yeast diploid cells, via multiple interstitial interactions, and independent of recA homologs and mating type heterozygosity. Pairing is present during G1 and G2, and in cells arrested at G1 by mating pheromone, but is disrupted during S phase. Thus, somatic pairing is qualitatively analogous to premeiotic and early meiotic pairing. S-phase pairing disruption occurs by a complex intranuclear program involving regional, nucleus-wide, and temporal determinants. Pairing is also disrupted in two G2-arrest conditions (cdc13ts and nocodazole). Together these findings suggest that cell cycle signals may provoke pairing disruption by modulating underlying chromosome and/or chromatin structure. Whether the cell chooses to disrupt pairing contacts or not (e.g., S phase and G2 arrest, but not G1 arrest or normal G1 or G2), could be dictated by functional considerations involving homolog/sister discrimination.

MeSH Terms
Animals Cell Cycle Chromosomes, Fungal Drosophila G1 Phase G2 Phase Heterozygote In Situ Hybridization, Fluorescence Meiosis Mitosis Pheromones S Phase Saccharomyces cerevisiae/genetics
Chemicals
Pheromones
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burgess S M
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Kleckner N
Weiner B M
References (52)
52 references, click to expand
  1. Analysis of a circular derivative of Saccharomyces cerevisiae chromosome III: a physical map and identification and location of ARS elements.
    Genetics. 1991 Oct;129(2):343-57 PMID: 1683846
  2. Segregation of recombinant chromatids following mitotic crossing over in yeast.
    Genetics. 1991 Oct;129(2):359-69 PMID: 1660426
  3. Sister chromatids are preferred over homologs as substrates for recombinational repair in Saccharomyces cerevisiae.
    Genetics. 1992 Oct;132(2):387-402 PMID: 1427035
  4. Cell cycle arrest of cdc mutants and specificity of the RAD9 checkpoint.
    Genetics. 1993 May;134(1):63-80 PMID: 8514150
  5. Somatic pairing of centromeres and short arms of chromosome 15 in the hematopoietic and lymphoid system.
    Hum Genet. 1993 Dec;92(6):577-82 PMID: 8262518
  6. Chromosome condensation and sister chromatid pairing in budding yeast.
    J Cell Biol. 1994 May;125(3):517-30 PMID: 8175878
  7. Homologous pairing is reduced but not abolished in asynaptic mutants of yeast.
    J Cell Biol. 1994 Jun;125(6):1191-200 PMID: 8207053
  8. Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast.
    Cell. 1994 Jul 1;77(7):977-91 PMID: 8020104
  9. Somatic pairing, reduction and recombination: an evolutionary hypothesis of meiosis.
    Nature. 1969 Jun 28;222(5200):1275-6 PMID: 5789667
  10. The nucleotide sequence of the HIS4 region of yeast.
    Gene. 1982 Apr;18(1):47-59 PMID: 7049842
  11. Gene conversion at different points in the mitotic cycle of Saccharomyces cerevisiae.
    Mol Gen Genet. 1984;195(1-2):139-43 PMID: 6387388
  12. The synaptonemal complex in genetic segregation.
    Annu Rev Genet. 1984;18:331-413 PMID: 6241453
  13. A Tn10-lacZ-kanR-URA3 gene fusion transposon for insertion mutagenesis and fusion analysis of yeast and bacterial genes.
    Genetics. 1987 Jun;116(2):191-9 PMID: 3038670
  14. Time of replication of yeast centromeres and telomeres.
    Cell. 1988 Aug 12;54(4):505-13 PMID: 3042152
  15. Double in situ hybridization in combination with digital image analysis: a new approach to study interphase chromosome topography.
    Exp Cell Res. 1989 Mar;181(1):126-40 PMID: 2917599
  16. Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum.
    Hum Genet. 1989 Oct;83(3):231-4 PMID: 2793166
  17. Three-dimensional analysis of the organization of human chromosome domains in human and human-hamster hybrid interphase nuclei.
    J Cell Sci. 1989 Oct;94 ( Pt 2):299-306 PMID: 2621226
  18. Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination.
    Cell. 1990 May 4;61(3):419-36 PMID: 2185891
  19. A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae.
    Cell. 1990 Jun 15;61(6):1089-101 PMID: 2190690
  20. Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast.
    J Cell Sci. 1990 Nov;97 ( Pt 3):509-16 PMID: 2074269
  21. Temporal comparison of recombination and synaptonemal complex formation during meiosis in S. cerevisiae.
    Cell. 1991 Sep 20;66(6):1239-56 PMID: 1913808
  22. Potential advantages of unstable interactions for pairing of chromosomes in meiotic, somatic, and premeiotic cells.
    Cold Spring Harb Symp Quant Biol. 1993;58:553-65 PMID: 7956070
  23. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.
    Yeast. 1995 Jan;11(1):53-5 PMID: 7762301
  24. Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint.
    Mol Cell Biol. 1995 Nov;15(11):6128-38 PMID: 7565765
  25. Heteroduplex DNA formation and homolog pairing in yeast meiotic mutants.
    Genetics. 1995 Sep;141(1):75-86 PMID: 8536992
  26. Roles for two RecA homologs in promoting meiotic chromosome synapsis.
    Genes Dev. 1995 Nov 1;9(21):2684-95 PMID: 7590245
  27. Homologous association of oppositely imprinted chromosomal domains.
    Science. 1996 May 3;272(5262):725-8 PMID: 8614834
  28. Multiple determinants controlling activation of yeast replication origins late in S phase.
    Genes Dev. 1996 Jul 1;10(13):1595-607 PMID: 8682291
  29. Meiotic transvection in fungi.
    Cell. 1996 Jul 12;86(1):103-13 PMID: 8689677
  30. A meiotic recombination checkpoint controlled by mitotic checkpoint genes.
    Nature. 1996 Oct 31;383(6603):840-3 PMID: 8893012
  31. The spindle assembly checkpoint.
    Curr Opin Cell Biol. 1996 Dec;8(6):773-80 PMID: 8939672
  32. Meiotic cells monitor the status of the interhomolog recombination complex.
    Genes Dev. 1997 Jan 1;11(1):106-18 PMID: 9000054
  33. Identification of a mid-anaphase checkpoint in budding yeast.
    J Cell Biol. 1997 Jan 27;136(2):345-54 PMID: 9015305
  34. Communication between homologous chromosomes: genetic alterations at a nuclease-hypersensitive site can alter mitotic chromatin structure at that site both in cis and in trans.
    Genes Cells. 1996 May;1(5):475-89 PMID: 9078379
  35. Interactions between and along chromosomes during meiosis.
    Harvey Lect. 1995-1996;91:21-45 PMID: 9127984
  36. Centromere position in budding yeast: evidence for anaphase A.
    Mol Biol Cell. 1997 Jun;8(6):957-72 PMID: 9201708
  37. Mitosis in living budding yeast: anaphase A but no metaphase plate.
    Science. 1997 Jul 25;277(5325):574-8 PMID: 9228009
  38. Paramutation and related allelic interactions.
    Trends Genet. 1997 Aug;13(8):302-8 PMID: 9260515
  39. Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway.
    Cell. 1997 Sep 19;90(6):1123-35 PMID: 9323140
  40. Intergenic transcription and transinduction of the human beta-globin locus.
    Genes Dev. 1997 Oct 1;11(19):2494-509 PMID: 9334315
  41. Interphase chromosomes undergo constrained diffusional motion in living cells.
    Curr Biol. 1997 Dec 1;7(12):930-9 PMID: 9382846
  42. The case for epigenetic effects on centromere identity and function.
    Trends Genet. 1997 Dec;13(12):489-96 PMID: 9433139
  43. Cdc7 is required throughout the yeast S phase to activate replication origins.
    Genes Dev. 1998 Feb 15;12(4):491-501 PMID: 9472018
  44. Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations.
    J Cell Biol. 1998 Apr 6;141(1):5-20 PMID: 9531544
  45. Yeast nuclei display prominent centromere clustering that is reduced in nondividing cells and in meiotic prophase.
    J Cell Biol. 1998 Apr 6;141(1):21-9 PMID: 9531545
  46. Suppression of crossing-over by DNA methylation in Ascobolus.
    Genes Dev. 1998 May 1;12(9):1381-9 PMID: 9573054
  47. Trans-sensing effects: the ups and downs of being together.
    Cell. 1998 May 1;93(3):329-32 PMID: 9590167
  48. Recombination at work for meiosis.
    Curr Opin Genet Dev. 1998 Apr;8(2):200-11 PMID: 9610411
  49. Cytosine methylation of repeated sequences in eukaryotes: the role of DNA pairing.
    Trends Biochem Sci. 1998 Jul;23(7):252-6 PMID: 9697415
  50. Large-scale chromosomal movements during interphase progression in Drosophila.
    J Cell Biol. 1998 Oct 5;143(1):13-22 PMID: 9763417
  51. Homologous association of the Bithorax-Complex during embryogenesis: consequences for transvection in Drosophila melanogaster.
    Development. 1998 Nov;125(22):4541-52 PMID: 9778512
  52. Separating sister chromatids.
    Trends Biochem Sci. 1999 Mar;24(3):98-104 PMID: 10203756
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.12.1627
Published
1999-06-15
Pages
1627-41
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316803
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044794 · United States
NIGMS NIH HHS · R01-GM44794 · United States
External Links
PubMed source DOI: 10.1101/gad.13.12.1627 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2