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PMID: 9573054 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Suppression of crossing-over by DNA methylation in Ascobolus.

Genes & development ·Vol. 12 ·No. 9 ·1998-05-01 ·Pages 1381-9

Maloisel L, Rossignol JL

Abstract

Homologous recombination between dispersed DNA repeats creates chromosomal rearrangements that are deleterious to the genome. The methylation associated with DNA repeats in many eukaryotes might serve to inhibit homologous recombination and play a role in preserving genome integrity. We have tested the hypothesis that DNA methylation suppresses meiotic recombination in the fungus Ascobolus immersus. The natural process of methylation-induced premeiotically (MIP) was used to methylate the b2 spore color gene, a 7.5-kb chromosomal recombination hot spot. The frequency of crossing-over between two markers flanking b2 was reduced several hundredfold when b2 was methylated on the two homologs. This demonstrates that DNA methylation strongly inhibits homologous recombination. When b2 was methylated on one homolog only, crossing-over was still reduced 50-fold, indicating that the effect of methylation cannot be limited to the blocking of initiation of recombination on the methylated homolog. On the basis of these and other observations, we propose that DNA methylation perturbs pairing between the two intact homologs before recombination initiation and/or impairs the normal processing of recombination intermediates.

MeSH Terms
Alleles Ascomycota/genetics,metabolism Crossing Over, Genetic DNA Methylation DNA, Fungal/chemistry,genetics,metabolism Gene Rearrangement Genetic Markers Meiosis/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Fungal Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maloisel L
Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France.
Rossignol J L
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.9.1381
Published
1998-05-01
Pages
1381-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316785
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.9.1381 PMC Full Text View journal details
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