Home LiteratureArticle Details
PMID: 8536992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heteroduplex DNA formation and homolog pairing in yeast meiotic mutants.

Genetics ·Vol. 141 ·No. 1 ·1995-09-00 ·Pages 75-86

Nag DK, Scherthan H, Rockmill B, Bhargava J, Roeder GS

Abstract

Previous studies of Saccharomyces cerevisiae have identified several meiosis-specific genes whose products are required for wild-type levels of meiotic recombination and for normal synaptonemal complex (SC) formation. Several of these mutants were examined in a physical assay designed to detect heteroduplex DNA (hDNA) intermediates in meiotic recombination. hDNA was not detected in the rec102, mei4 and hop1 mutants; it was observed at reduced levels in red1, mek1 and mer1 strains and at greater than the wild-type level in zip1. These results indicate that the REC102, MEI4, HOP1, RED1, MEK1 and MER1 gene products act before hDNA formation in the meiotic recombination pathway, whereas ZIP1 acts later. The same mutants assayed for hDNA formation were monitored for meiotic chromosome pairing by in situ hybridization of chromosome-specific DNA probes to spread meiotic nuclei. Homolog pairing occurs at wild-type levels in the zip1 and mek1 mutants, but is substantially reduced in mei4, rec102, hop1, red1 and mer1 strains. Even mutants that fail to recombine or to make any SC or SC precursors undergo a significant amount of meiotic chromosome pairing. The in situ hybridization procedure revealed defects in meiotic chromatin condensation in mer1, red1 and hop1 strains.

MeSH Terms
Chromosomes, Fungal DNA, Fungal Meiosis/genetics Mutation Nucleic Acid Heteroduplexes Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal Nucleic Acid Heteroduplexes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nag D K
Molecular Genetics Program, David Axelrod Institute, Wadsworth Center for Laboratories and Research, Albany, New York 12201, USA.
Scherthan H
Rockmill B
Bhargava J
Roeder G S
References (49)
49 references, click to expand
  1. Molecular and genetic analysis of the yeast early meiotic recombination genes REC102 and REC107/MER2.
    Mol Cell Biol. 1992 Mar;12(3):1248-56 PMID: 1545806
  2. The yeast MER2 gene is required for chromosome synapsis and the initiation of meiotic recombination.
    Genetics. 1995 Sep;141(1):49-59 PMID: 8536989
  3. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
    Cell. 1992 May 1;69(3):439-56 PMID: 1581960
  4. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.
    Cell. 1992 May 1;69(3):457-70 PMID: 1581961
  5. Multiple sites for double-strand breaks in whole meiotic chromosomes of Saccharomyces cerevisiae.
    EMBO J. 1992 Sep;11(9):3441-7 PMID: 1324174
  6. Mixed segregation and recombination of chromosomes and YACs during single-division meiosis in spo13 strains of Saccharomyces cerevisiae.
    Genetics. 1993 Oct;135(2):297-308 PMID: 8243995
  7. XRS2, a DNA repair gene of Saccharomyces cerevisiae, is needed for meiotic recombination.
    Genetics. 1992 Nov;132(3):651-64 PMID: 1468624
  8. Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase.
    Mol Cell Biol. 1993 Jan;13(1):373-82 PMID: 8417336
  9. ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis.
    Cell. 1993 Feb 12;72(3):365-78 PMID: 7916652
  10. Physical detection of heteroduplexes during meiotic recombination in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Apr;13(4):2324-31 PMID: 8455614
  11. A conditional allele of the Saccharomyces cerevisiae HOP1 gene is suppressed by overexpression of two other meiosis-specific genes: RED1 and REC104.
    Genetics. 1993 Apr;133(4):785-97 PMID: 8462842
  12. Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis.
    J Cell Biol. 1993 Apr;121(2):241-56 PMID: 8468345
  13. Mutations in U1 snRNA bypass the requirement for a cell type-specific RNA splicing factor.
    Cell. 1993 Apr 23;73(2):407-15 PMID: 8477451
  14. Genetic recombination and commitment to meiosis in Saccharomyces.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):3172-6 PMID: 4606582
  15. Recombination and chromosome segregation during the single division meiosis in SPO12-1 and SPO13-1 diploids.
    Genetics. 1980 Nov;96(3):589-611 PMID: 7021312
  16. The synaptonemal complex in genetic segregation.
    Annu Rev Genet. 1984;18:331-413 PMID: 6241453
  17. Fungal recombination.
    Microbiol Rev. 1985 Mar;49(1):33-58 PMID: 3884994
  18. Analysis of meiosis-defective mutations in yeast by physical monitoring of recombination.
    Genetics. 1986 Jul;113(3):551-67 PMID: 3015718
  19. Meiotic chromosome behavior in spread preparations of yeast.
    J Cell Biol. 1988 Mar;106(3):567-73 PMID: 2450094
  20. RED1: a yeast gene required for the segregation of chromosomes during the reductional division of meiosis.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6057-61 PMID: 3413075
  21. Double-strand breaks at an initiation site for meiotic gene conversion.
    Nature. 1989 Mar 2;338(6210):87-90 PMID: 2645528
  22. Chromatin organization at meiosis.
    Bioessays. 1988 Nov;9(5):151-3 PMID: 3071365
  23. HOP1: a yeast meiotic pairing gene.
    Genetics. 1989 Mar;121(3):445-62 PMID: 2653960
  24. Yeast mer1 mutants display reduced levels of meiotic recombination.
    Genetics. 1989 Feb;121(2):237-47 PMID: 2659434
  25. Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast.
    Nature. 1989 Jul 27;340(6231):318-20 PMID: 2546083
  26. MEI4, a yeast gene required for meiotic recombination.
    Genetics. 1989 Dec;123(4):675-82 PMID: 2693205
  27. Use of a ring chromosome and pulsed-field gels to study interhomolog recombination, double-strand DNA breaks and sister-chromatid exchange in yeast.
    Genetics. 1989 Dec;123(4):695-713 PMID: 2693206
  28. The HOP1 gene encodes a meiosis-specific component of yeast chromosomes.
    Cell. 1990 Apr 6;61(1):73-84 PMID: 2107981
  29. MER1, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis.
    Mol Cell Biol. 1990 May;10(5):2379-89 PMID: 2183032
  30. Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination.
    Cell. 1990 May 4;61(3):419-36 PMID: 2185891
  31. A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae.
    Cell. 1990 Jun 15;61(6):1089-101 PMID: 2190690
  32. Meiosis in asynaptic yeast.
    Genetics. 1990 Nov;126(3):563-74 PMID: 2249756
  33. Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site.
    Cell. 1991 Mar 22;64(6):1155-61 PMID: 2004421
  34. Meiotic chromosome synapsis in a haploid yeast.
    Chromosoma. 1991 May;100(4):221-8 PMID: 2055133
  35. Isolation of mutants defective in early steps of meiotic recombination in the yeast Saccharomyces cerevisiae.
    Genetics. 1991 May;128(1):79-88 PMID: 2060778
  36. Meiosis-specific RNA splicing in yeast.
    Cell. 1991 Sep 20;66(6):1257-68 PMID: 1840507
  37. The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis.
    Genetics. 1992 Jan;130(1):59-69 PMID: 1732169
  38. The MRE4 gene encodes a novel protein kinase homologue required for meiotic recombination in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1992 Feb 11;20(3):449-57 PMID: 1741279
  39. Identification of joint molecules that form frequently between homologs but rarely between sister chromatids during yeast meiosis.
    Cell. 1994 Jan 14;76(1):51-63 PMID: 8287479
  40. Meiosis-specific formation of joint DNA molecules containing sequences from homologous chromosomes.
    Cell. 1994 Jan 14;76(1):65-75 PMID: 8287480
  41. Meiosis-induced double-strand break sites determined by yeast chromatin structure.
    Science. 1994 Jan 28;263(5146):515-8 PMID: 8290959
  42. Insertional mutations in the yeast HOP1 gene: evidence for multimeric assembly in meiosis.
    Genetics. 1994 Feb;136(2):449-64 PMID: 8150275
  43. Homologous pairing is reduced but not abolished in asynaptic mutants of yeast.
    J Cell Biol. 1994 Jun;125(6):1191-200 PMID: 8207053
  44. Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast.
    Cell. 1994 Jul 1;77(7):977-91 PMID: 8020104
  45. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae.
    Genes Dev. 1994 May 1;8(9):1087-105 PMID: 7926789
  46. Potential advantages of unstable interactions for pairing of chromosomes in meiotic, somatic, and premeiotic cells.
    Cold Spring Harb Symp Quant Biol. 1993;58:553-65 PMID: 7956070
  47. Zip1-induced changes in synaptonemal complex structure and polycomplex assembly.
    J Cell Biol. 1995 Feb;128(4):455-66 PMID: 7860625
  48. Binding of a cell-type-specific RNA splicing factor to its target regulatory sequence.
    Mol Cell Biol. 1995 Apr;15(4):1953-60 PMID: 7891689
  49. MEI4, a meiosis-specific yeast gene required for chromosome synapsis.
    Mol Cell Biol. 1992 Mar;12(3):1340-51 PMID: 1545815
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/141.1.75
Published
1995-09-00
Pages
75-86
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206742
Subset
IM
Grants
NIGMS NIH HHS · GM-28904 · United States
External Links
PubMed source DOI: 10.1093/genetics/141.1.75 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2