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PMID: 8243995 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Mixed segregation and recombination of chromosomes and YACs during single-division meiosis in spo13 strains of Saccharomyces cerevisiae.

Genetics ·Vol. 135 ·No. 2 ·1993-10-00 ·Pages 297-308

Hugerat Y, Simchen G

Abstract

Diploid yeast strains, homozygous for the mutation spo13, undergo a single-division meiosis and form dyads (two spores held together in one ascus). Dyad analysis of spo13/spo13 strains with centromere-linked markers on five different chromosomes and on a pair of human DNA YACs shows that: (a) in spo13 meiosis, chromosomes undergo mixed segregation, namely some chromosomes segregate reductionally whereas others, in the same cell, segregate equationally; (b) different chromosomes exhibit different segregation tendencies; (c) recombination between homologous chromosomes might not determine that a bivalent undergoes reductional rather than equational segregation.

Related Genes
spo13
MeSH Terms
Cell Division Centromere/ultrastructure Chromosome Mapping Chromosomes, Artificial, Yeast Chromosomes, Fungal/ultrastructure Gene Conversion Genes, Fungal Genetic Markers Genotype Meiosis Mutation Recombination, Genetic Restriction Mapping Saccharomyces cerevisiae/cytology,genetics
Chemicals
Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hugerat Y
Department of Genetics, Hebrew University of Jerusalem, Israel.
Simchen G
References (27)
27 references, click to expand
  1. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  2. Isolation of a yeast centromere and construction of functional small circular chromosomes.
    Nature. 1980 Oct 9;287(5782):504-9 PMID: 6999364
  3. Internuclear transfer of genetic information in kar1-1/KAR1 heterokaryons in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Mar;1(3):245-53 PMID: 6765600
  4. DIS1: a yeast gene required for proper meiotic chromosome disjunction.
    Genetics. 1988 Jun;119(2):261-72 PMID: 3294101
  5. Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE.
    Genetics. 1979 Sep;93(1):51-65 PMID: 17248969
  6. Recombination and chromosome segregation during the single division meiosis in SPO12-1 and SPO13-1 diploids.
    Genetics. 1980 Nov;96(3):589-611 PMID: 7021312
  7. Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination.
    Cell. 1990 May 4;61(3):419-36 PMID: 2185891
  8. Mixed segregation of chromosomes during single-division meiosis of Saccharomyces cerevisiae.
    Genetics. 1990 Jul;125(3):475-85 PMID: 2199318
  9. Meiosis in haploid yeast.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2986-90 PMID: 7045878
  10. Centromeric regions control autonomous segregation tendencies in single-division meiosis of Saccharomyces cerevisiae.
    Genetics. 1990 Jul;125(3):487-94 PMID: 2199319
  11. Recombinationless meiosis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Oct;1(10):891-901 PMID: 7050657
  12. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
    Genetics. 1987 Aug;116(4):541-5 PMID: 3305158
  13. Diploid spore formation and other meiotic effects of two cell-division-cycle mutations of Saccharomyces cerevisiae.
    Genetics. 1980 Dec;96(4):859-76 PMID: 7021319
  14. Crossing over and Diploid Egg Formation in the Elongate Mutant of Maize.
    Genetics. 1975 Mar;79(3):435-50 PMID: 17248678
  15. Biochemical Mutants in the Smut Fungus Ustilago Maydis.
    Genetics. 1949 Sep;34(5):607-26 PMID: 17247336
  16. MER1, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis.
    Mol Cell Biol. 1990 May;10(5):2379-89 PMID: 2183032
  17. Role of conserved sequence elements in yeast centromere DNA.
    EMBO J. 1985 Jul;4(7):1867-74 PMID: 2992949
  18. Initiation of meiosis in cell cycle initiation mutants of Saccharomyces cerevisiae.
    Exp Cell Res. 1978 Mar 15;112(2):241-8 PMID: 344051
  19. Analysis of chromosome segregation in Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:749-73 PMID: 2005822
  20. Monitoring meiosis and sporulation in Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:94-110 PMID: 2005827
  21. Mutations in CEN3 cause aberrant chromosome segregation during meiosis in Saccharomyces cerevisiae.
    Genetics. 1989 Mar;121(3):477-89 PMID: 2653962
  22. Genetic control of chromosome synapsis in yeast meiosis.
    Genome. 1989;31(1):88-94 PMID: 2687110
  23. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.
    Mol Gen Genet. 1984;197(2):345-6 PMID: 6394957
  24. Polyamines eliminate an extreme size bias against transformation of large yeast artificial chromosome DNA.
    Genomics. 1991 May;10(1):10-6 PMID: 2045094
  25. The yeast RME1 gene encodes a putative zinc finger protein that is directly repressed by a1-alpha 2.
    Genes Dev. 1991 Nov;5(11):1982-9 PMID: 1936989
  26. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  27. HOP1: a yeast meiotic pairing gene.
    Genetics. 1989 Mar;121(3):445-62 PMID: 2653960
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/135.2.297
Published
1993-10-00
Pages
297-308
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205636
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/135.2.297 PMC Full Text View journal details
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