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PMID: 11134289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization of immature human immunodeficiency virus type 1.

Journal of virology ·Vol. 75 ·No. 2 ·2001-01-00 ·Pages 759-71

Wilk T, Gross I, Gowen BE, Rutten T, de Haas F, Welker R, Kräusslich HG, Boulanger P, Fuller SD

Abstract

Immature retrovirus particles contain radially arranged Gag polyproteins in which the N termini lie at the membrane and the C termini extend toward the particle's center. We related image features to the polyprotein domain structure by combining mutagenesis with cryoelectron microscopy and image analysis. The matrix (MA) domain appears as a thin layer tightly associated with the inner face of the viral membrane, separated from the capsid (CA) layer by a low-density region corresponding to its C terminus. Deletion of the entire p6 domain has no effect on the width or spacing of the density layers, suggesting that p6 is not ordered in immature human immunodeficiency virus type 1 (HIV-1). In vitro assembly of a recombinant Gag polyprotein containing only capsid (CA) and nucleocapsid (NC) domains results in the formation of nonenveloped spherical particles which display two layers with density matching that of the CA-NC portion of immature HIV-1 Gag particles. Authentic, immature HIV-1 displays additional surface features and an increased density between the lipid bilayers which reflect the presence of gp41. The other internal features match those of virus-like particles.

MeSH Terms
Capsid/chemistry Cell Membrane/metabolism Cryoelectron Microscopy Gene Deletion Gene Products, gag/chemistry,genetics,metabolism HIV-1/chemistry,genetics,physiology,ultrastructure Humans Image Processing, Computer-Assisted Lipid Bilayers Nucleocapsid/chemistry Protein Structure, Tertiary Recombinant Proteins/metabolism Viral Matrix Proteins/chemistry,genetics Virion/chemistry,ultrastructure Virus Assembly gag Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, gag Lipid Bilayers Recombinant Proteins Viral Matrix Proteins gag Gene Products, Human Immunodeficiency Virus p6 gag protein, Human immunodeficiency virus 1
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Wilk T
The Structural Biology Programme, European Molecular Biology Laboratory, D69012 Heidelberg, Federal Republic of Germany.
Gross I
Gowen B E
Rutten T
de Haas F
Welker R
Kräusslich H G
Boulanger P
Fuller S D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.75.2.759-771.2001
Published
2001-01-00
Pages
759-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC113972
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.75.2.759-771.2001 PMC Full Text View journal details
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