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PMID: 11134515 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effects of replication termination mutants on chromosome partitioning in Bacillus subtilis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 1 ·2001-01-02 ·Pages 212-7

Lemon KP, Kurtser I, Grossman AD

Abstract

Many circular genomes have replication termination systems, yet disruption of these systems does not cause an obvious defect in growth or viability. We have found that the replication termination system of Bacillus subtilis contributes to accurate chromosome partitioning. Partitioning of the terminus region requires that chromosome dimers, that have formed as a result of RecA-mediated homologous recombination, be resolved to monomers by the site-specific recombinase encoded by ripX. In addition, the chromosome must be cleared from the region of formation of the division septum. This process is facilitated by the spoIIIE gene product which is required for movement of a chromosome out of the way of the division septum during sporulation. We found that deletion of rtp, which encodes the replication termination protein, in combination with mutations in ripX or spoIIIE, led to an increase in production of anucleate cells. This increase in production of anucleate cells depended on recA, indicating that there is probably an increase in chromosome dimer formation in the absence of the replication termination system. Our results also indicate that SpoIIIE probably enhances the function of the RipX recombinase system. We also determined the subcellular location of the replication termination protein and found that it is a good marker for the position of the chromosome terminus.

MeSH Terms
Bacillus subtilis/cytology,genetics Bacterial Proteins/genetics,metabolism,physiology Cell Cycle Proteins/genetics,metabolism Cell Nucleus/physiology Chromosome Segregation Chromosomes, Bacterial/genetics DNA Nucleotidyltransferases/genetics,metabolism DNA Replication/genetics DNA-Binding Proteins/genetics,metabolism Dimerization Genes, Bacterial/genetics Integrases Microscopy, Fluorescence Mutation/genetics Rec A Recombinases/genetics,metabolism Recombinant Fusion Proteins Recombinases Recombination, Genetic Sigma Factor Transcription Factors
Chemicals
Bacterial Proteins Cell Cycle Proteins DNA-Binding Proteins Recombinant Fusion Proteins Recombinases SMC protein, Bacteria Sigma Factor Transcription Factors rtP protein, Bacillus subtilis spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus DNA Nucleotidyltransferases Integrases Rec A Recombinases integron integrase IntI1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lemon K P
Department of Biology, Building 68-530, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Kurtser I
Grossman A D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.011506098
Published
2001-01-02
Pages
212-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC14570
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041934 · United States
NIGMS NIH HHS · R37 GM041934 · United States
NIGMS NIH HHS · GM41934 · United States
External Links
PubMed source DOI: 10.1073/pnas.011506098 PMC Full Text View journal details
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