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PMID: 11149940 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Essential role of the small GTPase Rac in disease resistance of rice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 2 ·2001-01-16 ·Pages 759-64

Ono E, Wong HL, Kawasaki T, Hasegawa M, Kodama O, Shimamoto K

Abstract

Production of reactive oxygen intermediates (ROI) and a form of programmed cell death called hypersensitive response (HR) are often associated with disease resistance of plants. We have previously shown that the Rac homolog of rice, OsRac1, is a regulator of ROI production and cell death in rice. Here we show that the constitutively active OsRac1 (i) causes HR-like responses and greatly reduces disease lesions against a virulent race of the rice blast fungus; (ii) causes resistance against a virulent race of bacterial blight; and (iii) causes enhanced production of a phytoalexin and alters expression of defense-related genes. The dominant-negative OsRac1 suppresses elicitor-induced ROI production in transgenic cell cultures, and in plants suppresses the HR induced by the avirulent race of the fungus. Taken together, our findings strongly suggest that OsRac1 has a general role in disease resistance of rice.

MeSH Terms
Agrobacterium tumefaciens/genetics Gene Expression Regulation, Plant Immunity, Innate/genetics Magnaporthe/physiology Oryza/enzymology,genetics Plant Diseases/genetics,microbiology Plant Extracts/biosynthesis,genetics Plant Leaves/microbiology Plant Proteins/biosynthesis,genetics,physiology Plants, Genetically Modified Protoplasts/metabolism Recombinant Fusion Proteins/biosynthesis Sesquiterpenes Terpenes Transformation, Genetic rac1 GTP-Binding Protein/biosynthesis,genetics,physiology
Chemicals
Plant Extracts Plant Proteins Recombinant Fusion Proteins Sesquiterpenes Terpenes phytoalexins rac1 GTP-Binding Protein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ono E
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Wong H L
Kawasaki T
Hasegawa M
Kodama O
Shimamoto K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.021273498
Published
2001-01-16
Epub
2001-00-09
Pages
759-64
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC14661
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.021273498 PMC Full Text View journal details
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