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PMID: 9861059 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 26 ·1998-12-22 ·Pages 15849-54

Boyes DC, Nam J, Dangl JL

Abstract

Disease resistance in plants is often controlled by a gene-for-gene mechanism in which avirulence (avr) gene products encoded by pathogens are specifically recognized, either directly or indirectly, by plant disease resistance (R) gene products. Members of the NBS-LRR class of R genes encode proteins containing a putative nucleotide binding site (NBS) and carboxyl-terminal leucine-rich repeats (LRRs). Generally, NBS-LRR proteins do not contain predicted transmembrane segments or signal peptides, suggesting they are soluble cytoplasmic proteins. RPM1 is an NBS-LRR protein from Arabidopsis thaliana that confers resistance to Pseudomonas syringae expressing either avrRpm1 or avrB. RPM1 protein was localized by using an epitope tag. In contrast to previous suggestions, RPM1 is a peripheral membrane protein that likely resides on the cytoplasmic face of the plasma membrane. Furthermore, RPM1 is degraded coincident with the onset of the hypersensitive response, suggesting a negative feedback loop controlling the extent of cell death and overall resistance response at the site of infection.

MeSH Terms
Arabidopsis/genetics,microbiology Arabidopsis Proteins Cell Membrane/metabolism Cytoplasm/metabolism Genetic Vectors Immunity, Innate Kinetics Membrane Proteins/genetics Plant Proteins/genetics,metabolism Pseudomonas/pathogenicity Recombinant Fusion Proteins/biosynthesis,metabolism Transformation, Genetic
Chemicals
Arabidopsis Proteins Membrane Proteins Plant Proteins RPM1 protein, Arabidopsis Recombinant Fusion Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boyes D C
Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA.
Nam J
Dangl J L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.26.15849
Published
1998-12-22
Pages
15849-54
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC28133
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.95.26.15849 PMC Full Text View journal details
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