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PMID: 11158736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cross-resistance between triclosan and antibiotics in Pseudomonas aeruginosa is mediated by multidrug efflux pumps: exposure of a susceptible mutant strain to triclosan selects nfxB mutants overexpressing MexCD-OprJ.

Antimicrobial agents and chemotherapy ·Vol. 45 ·No. 2 ·2001-02-00 ·Pages 428-32

Chuanchuen R, Beinlich K, Hoang TT, Becher A, Karkhoff-Schweizer RR, Schweizer HP

Abstract

Triclosan is an antiseptic frequently added to items as diverse as soaps, lotions, toothpaste, and many commonly used household fabrics and plastics. Although wild-type Pseudomonas aeruginosa expresses the triclosan target enoyl-acyl carrier protein reductase, it is triclosan resistant due to expression of the MexAB-OprM efflux system. Exposure of a susceptible Delta(mexAB-oprM) strain to triclosan selected multidrug-resistant bacteria at high frequencies. These bacteria hyperexpressed the MexCD-OprJ efflux system due to mutations in its regulatory gene, nfxB. The MICs of several drugs for these mutants were increased up to 500-fold, including the MIC of ciprofloxacin, which was increased 94-fold. Whereas the MexEF-OprN efflux system also participated in triclosan efflux, this antimicrobial was not a substrate for MexXY-OprM.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Anti-Infective Agents, Local/pharmacology Bacterial Outer Membrane Proteins/analysis,genetics Bacterial Proteins/genetics Drug Resistance, Microbial/genetics Genes, MDR/genetics Microbial Sensitivity Tests Molecular Sequence Data Mutation Pseudomonas aeruginosa/drug effects,metabolism Triclosan/pharmacology
Chemicals
Anti-Bacterial Agents Anti-Infective Agents, Local Bacterial Outer Membrane Proteins Bacterial Proteins Triclosan
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chuanchuen R
Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-1677, USA.
Beinlich K
Hoang T T
Becher A
Karkhoff-Schweizer R R
Schweizer H P
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.45.2.428-432.2001
Published
2001-02-00
Pages
428-32
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC90308
Subset
IM
Grants
NIGMS NIH HHS · GM56685 · United States
External Links
PubMed source DOI: 10.1128/AAC.45.2.428-432.2001 PMC Full Text View journal details
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