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PMID: 11172074 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis genomes uncoupled 5 (GUN5) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 4 ·2001-02-13 ·Pages 2053-8

Mochizuki N, Brusslan JA, Larkin R, Nagatani A, Chory J

Abstract

A plastid-derived signal plays an important role in the coordinated expression of both nuclear- and chloroplast-localized genes that encode photosynthesis-related proteins. Arabidopsis GUN (genomes uncoupled) loci have been identified as components of plastid-to-nucleus signal transduction. Unlike wild-type plants, gun mutants have nuclear Lhcb1 expression in the absence of chloroplast development. We observed a synergistic phenotype in some gun double-mutant combinations, suggesting there are at least two independent pathways in plastid-to-nucleus signal transduction. There is a reduction of chlorophyll accumulation in gun4 and gun5 mutant plants, and a gun4gun5 double mutant shows an albino phenotype. We cloned the GUN5 gene, which encodes the ChlH subunit of Mg-chelatase. We also show that gun2 and gun3 are alleles of the known photomorphogenic mutants, hy1 and hy2, which are required for phytochromobilin synthesis from heme. These findings suggest that certain perturbations of the tetrapyrrole biosynthetic pathway generate a signal from chloroplasts that causes transcriptional repression of nuclear genes encoding plastid-localized proteins. The comparison of mutant phenotypes of gun5 and another Mg-chelatase subunit (ChlI) mutant suggests a specific function for ChlH protein in the plastid-signaling pathway.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism,physiology Cell Nucleus/metabolism Cloning, Molecular Genome, Plant Lyases/metabolism Magnesium Molecular Sequence Data Mutagenesis Plant Proteins/genetics,metabolism Plastids/metabolism Signal Transduction/physiology
Chemicals
Plant Proteins Lyases magnesium chelatase Magnesium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mochizuki N
Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa, Kyoto 606-8502, Japan. [email protected]
Brusslan J A
Larkin R
Nagatani A
Chory J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.98.4.2053
Email
[email protected]
Published
2001-02-13
Pages
2053-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC29380
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.98.4.2053 PMC Full Text View journal details
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