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PMID: 11226176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sec63p and Kar2p are required for the translocation of SRP-dependent precursors into the yeast endoplasmic reticulum in vivo.

The EMBO journal ·Vol. 20 ·No. 1-2 ·2001-01-15 ·Pages 262-71

Young BP, Craven RA, Reid PJ, Willer M, Stirling CJ

Abstract

The translocation of secretory polypeptides into the endoplasmic reticulum (ER) occurs at the translocon, a pore-forming structure that orchestrates the transport and maturation of polypeptides at the ER membrane. In yeast, targeting of secretory precursors to the translocon can occur by two distinct pathways that are distinguished by their dependence upon the signal recognition particle (SRP). The SRP-dependent pathway requires SRP and its membrane-bound receptor, whereas the SRP-independent pathway requires a separate receptor complex consisting of Sec62p, Sec63p, Sec71p, Sec72p plus lumenal Kar2p/BiP. Here we demonstrate that Sec63p and Kar2p are also required for the SRP-dependent targeting pathway in vivo. Furthermore, we demonstrate multiple roles for Sec63p, at least one of which is exclusive to the SRP-independent pathway.

MeSH Terms
Cell Membrane/metabolism Endoplasmic Reticulum/metabolism Ethyl Methanesulfonate/pharmacology Fungal Proteins/metabolism Genotype HSP70 Heat-Shock Proteins/metabolism Heat-Shock Proteins/metabolism Membrane Proteins/metabolism Membrane Transport Proteins Methionine/metabolism Mutagenesis Protein Transport Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Signal Recognition Particle/metabolism
Chemicals
Fungal Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins KAR2 protein, yeast Membrane Proteins Membrane Transport Proteins SEC63 protein, S cerevisiae Saccharomyces cerevisiae Proteins Signal Recognition Particle Ethyl Methanesulfonate Methionine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Young B P
School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK.
Craven R A
Reid P J
Willer M
Stirling C J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.1.262
Published
2001-01-15
Pages
262-71
Language
English
Region
England
NLM ID
8208664
PMCID
PMC140194
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.1.262 PMC Full Text View journal details
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