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PMID: 8305736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum.

Molecular biology of the cell ·Vol. 4 ·No. 11 ·1993-11-00 ·Pages 1145-59

Scidmore MA, Okamura HH, Rose MD

Abstract

KAR2 encodes the yeast homologue of mammalian BiP, the endoplasmic reticulum (ER) resident member of the HSP70 family. Kar2p has been shown to be required for the translocation of proteins across the ER membrane as well as nuclear fusion. Sec63, an ER integral membrane protein that shares homology with the Escherichia coli DnaJ protein, is also required for translocation. In this paper we describe several specific genetic interactions between these two proteins, Kar2p and Sec63p. First, temperature-sensitive mutations in KAR2 and SEC63 form synthetic lethal combinations. Second, dominant mutations in KAR2 are allele-specific suppressors for the temperature-sensitive growth and translocation defect of sec63-1. Third, the sec63-1, unlike other translocation defective mutations, results in the induction of KAR2 mRNA levels. Taken together, these genetic interactions suggest that Kar2p and Sec63p interact in vivo in a manner similar to that of the E. coli HSP70, DnaK, and DnaJ. We propose that the interaction between these two proteins is critical to their function in protein translocation.

MeSH Terms
Crosses, Genetic Endoplasmic Reticulum/physiology Fungal Proteins/genetics,metabolism HSP70 Heat-Shock Proteins Heat-Shock Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Membrane Transport Proteins Mutation/genetics RNA, Fungal/analysis,genetics RNA, Messenger/analysis,genetics Radioimmunoassay Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Suppression, Genetic/genetics Temperature
Chemicals
Fungal Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins KAR2 protein, yeast Membrane Proteins Membrane Transport Proteins RNA, Fungal RNA, Messenger SEC63 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Scidmore M A
Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
Okamura H H
Rose M D
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.4.11.1145
Published
1993-11-00
Pages
1145-59
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275750
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037739 · United States
NIGMS NIH HHS · GM-37739 · United States
External Links
PubMed source DOI: 10.1091/mbc.4.11.1145 PMC Full Text View journal details
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