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PMID: 11266554 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression.

Nucleic acids research ·Vol. 29 ·No. 7 ·2001-04-01 ·Pages 1524-33

Stockinger EJ, Mao Y, Regier MK, Triezenberg SJ, Thomashow MF

Abstract

The ARABIDOPSIS CBF transcriptional activators bind to the CRT/DRE regulatory element present in the promoters of many cold-regulated genes and stimulate their transcription. Expression of the CBF1 proteins in yeast activates reporter genes carrying a minimal promoter with the CRT/DRE as an upstream regulatory element. Here we report that this ability of CBF1 is dependent upon the activities of three key components of the yeast Ada and SAGA complexes, namely the histone acetyltransferase (HAT) Gcn5 and the transcriptional adaptor proteins Ada2 and Ada3. This result suggested that CBF1 might function through the action of similar complexes in ARABIDOPSIS In support of this hypothesis we found that ARABIDOPSIS has a homolog of the GCN5 gene and two homologs of ADA2, the first report of multiple ADA2 genes in an organism. The ARABIDOPSIS GCN5 protein has intrinsic HAT activity and can physically interact in vitro with both the ARABIDOPSIS ADA2a and ADA2b proteins. In addition, the CBF1 transcriptional activator can interact with the ARABIDOPSIS GCN5 and ADA2 proteins. We conclude that ARABIDOPSIS encodes HAT-containing adaptor complexes that are related to the Ada and SAGA complexes of yeast and propose that the CBF1 transcriptional activator functions through the action of one or more of these complexes.

MeSH Terms
Acetyltransferases/genetics,metabolism Amino Acid Sequence Arabidopsis/enzymology,genetics Arabidopsis Proteins Cold Temperature DNA, Complementary/chemistry,genetics DNA, Plant/chemistry,genetics DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genes, Plant/genetics Histone Acetyltransferases Histones/metabolism Molecular Sequence Data Protein Binding RNA, Plant/genetics,metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Tissue Distribution Trans-Activators/genetics,metabolism Transcriptional Activation
Chemicals
Arabidopsis Proteins CBF1 protein, Arabidopsis DNA, Complementary DNA, Plant DNA-Binding Proteins Histones RNA, Plant Saccharomyces cerevisiae Proteins Trans-Activators Acetyltransferases Histone Acetyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stockinger E J
Department of Crop and Soil Sciences and Department of Biochemistry, Michigan State University, East Lansing, MI 48824, USA.
Mao Y
Regier M K
Triezenberg S J
Thomashow M F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/29.7.1524
Published
2001-04-01
Pages
1524-33
Language
English
Region
England
NLM ID
0411011
PMCID
PMC31267
Subset
IM
Databases
GENBANK
AF338768, AF338769, AF338770, AF338771
External Links
PubMed source DOI: 10.1093/nar/29.7.1524 PMC Full Text View journal details
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