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PMID: 9499399 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Histone acetyltransferase activity of yeast Gcn5p is required for the activation of target genes in vivo.

Genes & development ·Vol. 12 ·No. 5 ·1998-03-01 ·Pages 627-39

Kuo MH, Zhou J, Jambeck P, Churchill ME, Allis CD

Abstract

Gcn5p is a transcriptional coactivator required for correct expression of various genes in yeast. Several transcriptional regulators, including Gcn5p, possess intrinsic histone acetyltransferase (HAT) activity in vitro. However, whether the HAT activity of any of these proteins is required for gene activation remains unclear. Here, we demonstrate that the HAT activity of Gcn5p is critical for transcriptional activation of target genes in vivo. Core histones are hyperacetylated in cells overproducing functional Gcn5p, and promoters of Gcn5p-regulated genes are associated with hyperacetylated histones upon activation by low-copy Gcn5p. Point mutations within the Gcn5p catalytic domain abolish both promoter-directed histone acetylation and Gcn5p-mediated transcriptional activation. These data provide the first in vivo evidence that promoter-specific histone acetylation, catalyzed by functional Gcn5p, plays a critical role in gene activation.

MeSH Terms
Acetylation Acetyltransferases/metabolism Amino Acid Sequence Binding Sites Conserved Sequence Cytochrome c Group/genetics,metabolism Cytochromes c DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Histone Acetyltransferases Histones/metabolism Hydro-Lyases/genetics,metabolism Molecular Sequence Data Mutation Precipitin Tests Promoter Regions, Genetic Protein Kinases/genetics,metabolism Saccharomyces cerevisiae Proteins Trans-Activators/genetics,metabolism Transcription, Genetic Transcriptional Activation Yeasts/genetics,growth & development beta-Galactosidase/genetics,metabolism
Chemicals
CYC1 protein, S cerevisiae Cytochrome c Group DNA-Binding Proteins Fungal Proteins Histones Saccharomyces cerevisiae Proteins Trans-Activators Cytochromes c Acetyltransferases Histone Acetyltransferases Protein Kinases beta-Galactosidase Hydro-Lyases imidazoleglycerolphosphate dehydratase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kuo M H
Department of Biology, University of Rochester, Rochester, New York 14627, USA.
Zhou J
Jambeck P
Churchill M E
Allis C D
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.5.627
Published
1998-03-01
Pages
627-39
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316582
Subset
IM
Grants
NCRR NIH HHS · P41 RR012408 · United States
NCRR NIH HHS · P41 RR012408-080045 · United States
NIGMS NIH HHS · GM53512 · United States
NIGMS NIH HHS · GM52100 · United States
External Links
PubMed source DOI: 10.1101/gad.12.5.627 PMC Full Text View journal details
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