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PMID: 9154821 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3.

Molecular and cellular biology ·Vol. 17 ·No. 6 ·1997-06-00 ·Pages 3220-8

Horiuchi J, Silverman N, Piña B, Marcus GA, Guarente L

Abstract

The ADA genes encode factors which are proposed to function as transcriptional coactivators. Here we describe the cloning, sequencing, and initial characterization of a novel ADA gene, ADA1. Similar to the previously isolated ada mutants, ada1 mutants display decreases in transcription from various reporters. Furthermore, ADA1 interacts with the other ADAs in the ADA/GCN5 complex as demonstrated by partial purification of the complex and immunoprecipitation experiments. We estimate that the complex has a molecular mass of approximately 2 MDa. Previously, it had been demonstrated that ada5 mutants displayed more severe phenotypic defects than the other ada mutants (G. A. Marcus, J. Horiuchi, N. Silverman, and L. Guarente, Mol. Cell. Biol. 16:3197-3205, 1996; S. M. Roberts and F. Winston, Mol. Cell. Biol. 16:3206-3213, 1996). ada1 mutants display defects similar to those of ada5 mutants and different from those of the other mutants with respect to promoters affected, inositol auxotrophy, and Spt- phenotypes. Thus, the ADAs can be separated into two classes, suggesting that the ADA/GCN5 complex may have two separate functions. We present a speculative model on the possible roles of the ADA/GCN5 complex.

MeSH Terms
Acetylation Adaptor Proteins, Signal Transducing Blotting, Western Cloning, Molecular DNA, Fungal/chemistry DNA-Binding Proteins/metabolism Fungal Proteins/metabolism Histone Acetyltransferases Histones/metabolism Inositol/metabolism Macromolecular Substances Molecular Sequence Data Phenotype Protein Kinases/metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Trans-Activators/genetics,metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
ADA2 protein, S cerevisiae Adaptor Proteins, Signal Transducing DNA, Fungal DNA-Binding Proteins Fungal Proteins HFI1 protein, S cerevisiae Histones Macromolecular Substances NGG1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Inositol GCN5 protein, S cerevisiae Histone Acetyltransferases Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Horiuchi J
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Silverman N
Piña B
Marcus G A
Guarente L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.6.3220
Published
1997-06-00
Pages
3220-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232175
Subset
IM
Grants
NIGMS NIH HHS · GM50207 · United States
Databases
GENBANK
U41324, U76735
External Links
PubMed source DOI: 10.1128/MCB.17.6.3220 PMC Full Text View journal details
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