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PMID: 2643115 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Activation of yeast RNA polymerase II transcription by a thymidine-rich upstream element in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 2 ·1989-01-00 ·Pages 486-90

Lue NF, Buchman AR, Kornberg RD

Abstract

A thymidine-rich sequence upstream of the DED1 gene of Saccharomyces cerevisiae activated transcription of the CYC1 promoter by RNA polymerase II in vitro. Activation was inhibited by an excess of an oligonucleotide with the same but not a closely related thymidine-rich sequence, pointing to the involvement of a specific thymidine-rich element-binding factor. The extent of activation was as great as 30-fold and showed a similar distance and orientation dependence and a similar effect of deletions in vitro as in vivo.

MeSH Terms
Base Sequence DNA, Fungal/genetics Molecular Sequence Data Plasmids RNA Polymerase II/genetics Saccharomyces cerevisiae/enzymology,genetics Templates, Genetic Transcription, Genetic
Chemicals
DNA, Fungal RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lue N F
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
Buchman A R
Kornberg R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.2.486
Published
1989-01-00
Pages
486-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286495
Subset
IM
Grants
NIGMS NIH HHS · GM-36659 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.2.486 PMC Full Text View journal details
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