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PMID: 3321057 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8839-43

Lue NF, Kornberg RD

Abstract

A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

MeSH Terms
Acetates Cell-Free System Cloning, Molecular Genes, Fungal Promoter Regions, Genetic RNA Polymerase II/metabolism Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/metabolism Templates, Genetic Transcription Factors/metabolism Transcription, Genetic
Chemicals
Acetates Transcription Factors RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lue N F
Department of Cell Biology, Stanford University School of Medicine, CA 94305.
Kornberg R D
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.8839
Published
1987-12-00
Pages
8839-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299646
Subset
IM
Grants
NIGMS NIH HHS · GM-36659 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.24.8839 PMC Full Text View journal details
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