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PMID: 11285239 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of an RNA-protein complex involved in chloroplast group II intron trans-splicing in Chlamydomonas reinhardtii.

The EMBO journal ·Vol. 20 ·No. 7 ·2001-04-02 ·Pages 1765-73

Rivier C, Goldschmidt-Clermont M, Rochaix JD

Abstract

In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear loci and one chloroplast gene, tscA, are needed for this process. We have cloned Raa3, the first nuclear gene implicated in the splicing of intron 1. The predicted sequence of Raa3 consists of 1783 amino acids and shares a small region of homology with pyridoxamine 5'-phosphate oxidases. Raa3 is present in the soluble fraction of the chloroplast and is part of a large 1700 kDa complex, which also contains tscA RNA and the first psaA exon transcript. These partners, in association with other factors, form a chloroplast RNP particle that is required for the splicing of the first intron of psaA and which may be the counterpart of eukaryotic snRNPs involved in nuclear splicing.

MeSH Terms
Algal Proteins/genetics Amino Acid Sequence Animals Base Sequence Chlamydomonas reinhardtii/genetics,metabolism Chloroplast Proteins Chloroplasts/metabolism Cloning, Molecular DNA, Plant Exons Genes, Plant Introns Light-Harvesting Protein Complexes Molecular Sequence Data Nuclear Proteins/genetics,metabolism Photosynthetic Reaction Center Complex Proteins/genetics Photosystem I Protein Complex Plant Proteins/genetics Protozoan Proteins/genetics RNA, Plant Sequence Analysis, DNA Sequence Homology, Amino Acid Solubility Trans-Splicing
Chemicals
Algal Proteins Chloroplast Proteins DNA, Plant Light-Harvesting Protein Complexes Nuclear Proteins Photosynthetic Reaction Center Complex Proteins Photosystem I Protein Complex Plant Proteins Protozoan Proteins RNA, Plant tscA protein, Chlamydomonas
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rivier C
Departments of Molecular Biology and Plant Biology, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland.
Goldschmidt-Clermont M
Rochaix J D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.7.1765
Published
2001-04-02
Pages
1765-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC145512
Subset
IM
Databases
GENBANK
AF310674, AF310675
External Links
PubMed source DOI: 10.1093/emboj/20.7.1765 PMC Full Text View journal details
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