Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii.

Current genetics ·Vol. 2 ·No. 3 ·1980-12-00 ·Pages 215-21

Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M

Abstract

Genetic analysis of 25 nuclear mutants defective in the chlorophyll-protein complex CP1 was undertaken. The mutants belong to 13 complementation groups scattered throughout the nuclear genome. All these mutants lack the apoprotein of CP1 and, in addition, a specific set of six low molecular weight thylakoid polypeptides. System I particles obtained by treating WT thylakoid membranes with detergent specifically contain those polypeptides which the mutants lack. These observations suggest that a particular sub-structure of the thylakoid membrane associated with the photosystem I activity is missing from all 25 mutants studied, and that this general phenotype can result from mutation at any one of several unlinked Mendelian loci.

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Girard J

Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, F-75005, Paris, France.

Chua N H

Bennoun P

Schmidt G

Delosme M

References (14)

14 references, click to expand

The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi.
Annu Rev Genet. 1970;4:397-408 PMID: 4268904
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Chlamydomonas reinhardi: heterozygous diploid strains.
Science. 1967 Jul 28;157(3787):447-9 PMID: 6028032
Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.
Plant Physiol. 1967 Sep;42(9):1284-7 PMID: 16656650
The sites of synthesis of the principal thylakoid membrane polypeptides in Chlamydomonas reinhardtii.
J Cell Biol. 1977 Aug;74(2):441-52 PMID: 142091
A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii.
J Cell Biol. 1975 Nov;67(2PT.1):361-77 PMID: 1194353
A rapid procedure for selective enrichment of photosynthetic electron transport mutants.
Proc Natl Acad Sci U S A. 1977 Feb;74(2):610-4 PMID: 265526
Transport of proteins into mitochondria and chloroplasts.
J Cell Biol. 1979 Jun;81(3):461-83 PMID: 379018
Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175-9 PMID: 1056023
The genetics and cytology of Chlamydomonas.
Annu Rev Microbiol. 1960;14:197-216 PMID: 13761500
Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.
Anal Biochem. 1971 Feb;39(2):462-77 PMID: 4101989
Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.
Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665-9 PMID: 4379719
Subunit structure of chloroplast photosystem I reaction center.
J Biol Chem. 1977 Jul 10;252(13):4564-9 PMID: 17601
Chlorophyll proteins of photosystem I.
Plant Physiol. 1980 May;65(5):814-22 PMID: 16661288

Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00435689

Published

1980-12-00

Pages

215-21

Language

English

Region

United States

NLM ID

8004904

External Links

PubMed source DOI: 10.1007/BF00435689 View journal details

Analysis Services

Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii.

Contact

WeChat Official Account