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PMID: 11333232 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The G-protein beta-subunit GPB-2 in Caenorhabditis elegans regulates the G(o)alpha-G(q)alpha signaling network through interactions with the regulator of G-protein signaling proteins EGL-10 and EAT-16.

Genetics ·Vol. 158 ·No. 1 ·2001-05-00 ·Pages 221-35

van der Linden AM, Simmer F, Cuppen E, Plasterk RH

Abstract

The genome of Caenorhabditis elegans harbors two genes for G-protein beta-subunits. Here, we describe the characterization of the second G-protein beta-subunit gene gpb-2. In contrast to gpb-1, gpb-2 is not an essential gene even though, like gpb-1, gpb-2 is expressed during development, in the nervous system, and in muscle cells. A loss-of-function mutation in gpb-2 produces a variety of behavioral defects, including delayed egg laying and reduced pharyngeal pumping. Genetic analysis shows that GPB-2 interacts with the GOA-1 (homologue of mammalian G(o)alpha) and EGL-30 (homologue of mammalian G(q)alpha) signaling pathways. GPB-2 is most similar to the divergent mammalian Gbeta5 subunit, which has been shown to mediate a specific interaction with a Ggamma-subunit-like (GGL) domain of RGS proteins. We show here that GPB-2 physically and genetically interacts with the GGL-containing RGS proteins EGL-10 and EAT-16. Taken together, our results suggest that GPB-2 works in concert with the RGS proteins EGL-10 and EAT-16 to regulate GOA-1 (G(o)alpha) and EGL-30 (G(q)alpha) signaling.

MeSH Terms
Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans/metabolism Caenorhabditis elegans Proteins DNA Primers GTP-Binding Protein Regulators GTP-Binding Proteins/chemistry,metabolism Helminth Proteins/metabolism Phenotype RGS Proteins/metabolism Signal Transduction Two-Hybrid System Techniques
Chemicals
Caenorhabditis elegans Proteins DNA Primers EAT-16 protein, C elegans EGL-10 protein, C elegans GTP-Binding Protein Regulators Helminth Proteins RGS Proteins GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
van der Linden A M
Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Simmer F
Cuppen E
Plasterk R H
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/158.1.221
Published
2001-05-00
Pages
221-35
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461628
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/158.1.221 PMC Full Text View journal details
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