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PMID: 11353084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis.

Nucleic acids research ·Vol. 29 ·No. 10 ·2001-05-15 ·Pages 2145-53

Chambaud I, Heilig R, Ferris S, Barbe V, Samson D, Galisson F, Moszer I, Dybvig K, Wróblewski H, Viari A, Rocha EP, Blanchard A

Abstract

Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.

MeSH Terms
Animals Antigens, Bacterial/genetics,immunology Base Composition Codon, Terminator/genetics Computational Biology Evolution, Molecular Genetic Code Genome Genomic Library Humans Internet Lipoproteins/genetics Mice Molecular Sequence Data Mutation/genetics Mycoplasma/genetics,immunology,pathogenicity Open Reading Frames/genetics Polymorphism, Genetic/genetics RNA, Bacterial/genetics Recombination, Genetic/genetics Repetitive Sequences, Nucleic Acid/genetics Replication Origin/genetics Respiratory System/microbiology Virulence/genetics
Chemicals
Antigens, Bacterial Codon, Terminator Lipoproteins RNA, Bacterial
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Chambaud I
INRA-Université de Bordeaux 2, Institut de Biologie Végétale Moléculaire, 71 avenue Edouard Bourleaux, BP 81, 33883 Villenave D'Ornon Cedex, France.
Heilig R
Ferris S
Barbe V
Samson D
Galisson F
Moszer I
Dybvig K
Wróblewski H
Viari A
Rocha E P
Blanchard A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/29.10.2145
Published
2001-05-15
Pages
2145-53
Language
English
Region
England
NLM ID
0411011
PMCID
PMC55444
Subset
IM
Grants
NIAID NIH HHS · R01 AI041113 · United States
NIAID NIH HHS · R21 AI041113 · United States
NIAID NIH HHS · AI41113 · United States
Databases
GENBANK
AL445566
External Links
PubMed source DOI: 10.1093/nar/29.10.2145 PMC Full Text View journal details
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