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PMID: 11387220 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Requirement of Hsp90 for centrosomal function reflects its regulation of Polo kinase stability.

The EMBO journal ·Vol. 20 ·No. 11 ·2001-06-01 ·Pages 2878-84

de Cárcer G, do Carmo Avides M, Lallena MJ, Glover DM, González C

Abstract

We have previously shown that the molecular chaperone heat shock protein 90 (Hsp90) is required to ensure proper centrosome function in Drosophila and vertebrate cells. This observation led to the hypothesis that this chaperone could be required for the stability of one or more centrosomal proteins. We have found that one of these is Polo, a protein kinase known to regulate several aspects of cell division including centrosome maturation and function. Inhibition of Hsp90 results in the inactivation of Polo kinase activity. It also leads to a loss in the ability of cytoplasmic extracts to complement the failure of salt-stripped preparations of centrosomes to nucleate microtubules. This effect can be rescued upon addition of active recombinant POLO: We also show that Polo and Hsp90 are part of a complex and conclude that stabilization of Polo is one of the mechanisms by which Hsp90 contributes to the maintenance of functional centrosomes.

MeSH Terms
Animals Cell Division Cell Line Centrosome/physiology Drosophila Proteins Drosophila melanogaster/embryology,genetics Embryo, Nonmammalian/physiology Genetic Complementation Test HSP90 Heat-Shock Proteins/metabolism Protein Serine-Threonine Kinases/chemistry,metabolism Recombinant Proteins/metabolism Vertebrates
Chemicals
Drosophila Proteins HSP90 Heat-Shock Proteins Recombinant Proteins polo protein, Drosophila Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
de Cárcer G
Cell Biology and Biophysics, and Gene Expression Programmes, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
do Carmo Avides M
Lallena M J
Glover D M
González C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.11.2878
Published
2001-06-01
Pages
2878-84
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125474
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.11.2878 PMC Full Text View journal details
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