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PMID: 11404352 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A histone deacetylation inhibitor and mutant promote colony-type switching of the human pathogen Candida albicans.

Genetics ·Vol. 158 ·No. 2 ·2001-06-00 ·Pages 919-24

Klar AJ, Srikantha T, Soll DR

Abstract

Most strains of Candida albicans undergo high frequency phenotypic switching. Strain WO-1 undergoes the white-opaque transition, which involves changes in colony and cellular morphology, gene expression, and virulence. We have hypothesized that the switch event involves heritable changes in chromatin structure. To test this hypothesis, we transiently exposed cells to the histone deacetylase inhibitor trichostatin-A (TSA). Treatment promoted a dramatic increase in the frequency of switching from white to opaque, but not opaque to white. Targeted deletion of HDA1, which encodes a deacetylase sensitive to TSA, had the same selective effect. These results support the model that the acetylation of histones plays a selective role in regulating the switching process.

MeSH Terms
Antifungal Agents/pharmacology Candida albicans/drug effects,genetics,physiology Cell Division/drug effects Histone Deacetylase Inhibitors Hydroxamic Acids/pharmacology Mutation Phenotype Up-Regulation
Chemicals
Antifungal Agents Histone Deacetylase Inhibitors Hydroxamic Acids trichostatin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klar A J
Gene Regulation and Chromosome Biology Laboratory, National Cancer Institute at Frederick, DHHS, NCI, DBS, Frederick, MD 21702-1201, USA. [email protected]
Srikantha T
Soll D R
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/158.2.919
Email
[email protected]
Published
2001-06-00
Pages
919-24
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461676
Subset
IM
Grants
NIAID NIH HHS · AI-2392 · United States
External Links
PubMed source DOI: 10.1093/genetics/158.2.919 PMC Full Text View journal details
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