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PMID: 11404406 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NMDA receptor-mediated Na+ signals in spines and dendrites.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 21 ·No. 12 ·2001-06-15 ·Pages 4207-14

Rose CR, Konnerth A

Abstract

Spines and dendrites of central neurons represent an important site of synaptic signaling and integration. Here we identify a new, synaptically mediated spine signal with unique properties. Using two-photon Na(+) imaging, we show that suprathreshold synaptic stimulation leads to transient increases in Na(+) concentration in postsynaptic spines and their adjacent dendrites. This local signal is restricted to a dendritic domain near the site of synaptic input. In presumed active spines within this domain, the Na(+) level increases by 30-40 mm even during short bursts of synaptic stimulation. During a long-term potentiation induction protocol (100 Hz, 1 sec), the Na(+) level in the active spines reaches peak amplitudes of approximately 100 mm. We find that the Na(+) transients are mainly mediated by Na(+) entry through NMDA receptor channels and are detected during the coincident occurrence of synaptic potentials and backpropagating action potentials. The large amplitudes of the Na(+) transients and their location on dendritic spines suggest that this signal is an important determinant of electrical and biochemical spine characteristics.

MeSH Terms
Action Potentials/physiology Animals Cell Surface Extensions/metabolism Dendrites/metabolism Electric Stimulation/methods Hippocampus/cytology,metabolism In Vitro Techniques Long-Term Potentiation/physiology Membrane Potentials/physiology Mice Microscopy, Confocal Patch-Clamp Techniques Pyramidal Cells/metabolism Receptors, N-Methyl-D-Aspartate/metabolism Sensory Thresholds/physiology Signal Transduction/physiology Sodium/metabolism Synaptic Transmission/physiology
Chemicals
Receptors, N-Methyl-D-Aspartate Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rose C R
Institut für Physiologie, Ludwig-Maximilians-Universität München, D-80802 München, Germany. [email protected]
Konnerth A
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Email
[email protected]
Published
2001-06-15
Pages
4207-14
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6762772
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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