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PMID: 9204921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A long-lasting calcium-activated nonselective cationic current is generated by synaptic stimulation or exogenous activation of group I metabotropic glutamate receptors in CA1 pyramidal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 17 ·No. 14 ·1997-07-15 ·Pages 5366-79

Congar P, Leinekugel X, Ben-Ari Y, Crépel V

Abstract

We have shown previously that a selective metabotropic glutamate receptor (mGluR) agonist, 1S,3R-1-aminocyclo-pentane-1, 3-dicarboxylate (1S,3R-ACPD), evokes an inward current in CA1 pyramidal neurons of rat hippocampal slices in the presence of K+ channel blockers (). This current has been characterized as a Ca2+-activated nonselective cationic (CAN) current. Using whole-cell patch-clamp recordings and intracellular dialysis, we now have identified the mGluR subtype and the mechanisms underlying the CAN current (ICAN) and report for the first time the presence of a synaptic ICAN in the mammalian CNS. First, we have shown pharmacologically that activation of ICAN by 1S,3R-ACPD involves the group I mGluRs (and not the groups II and III) and a G-protein-dependent process. We also report that ICAN is modulated by the divalent cations (Mg2+, Cd2+, and Zn2+). Second, we have isolated a slow synaptic inward current evoked by a high-frequency stimulation in the presence of K+ channel blockers, ionotropic glutamate, and GABAA receptor antagonists. This current shows similar properties to the exogenously evoked ICAN: its reversal potential is close to the reversal potential of the 1S, 3R-ACPD-evoked ICAN, and it is G-protein- and Ca2+-dependent. Because the amplitude and duration of ICAN increased in the presence of a glutamate uptake blocker, we suggest that this synaptic current is generated via the activation of mGluRs. We propose that the synaptic ICAN, activated by a brief tetanic stimulation and leading to a long-lasting inward current, may be involved in neuronal plasticity and synchronized network-driven oscillations.

MeSH Terms
Animals Calcium/pharmacology Cations/metabolism Cycloleucine/analogs & derivatives,pharmacology Ion Transport/drug effects Male Neuroprotective Agents/pharmacology Patch-Clamp Techniques Pyramidal Cells/drug effects Rats Rats, Wistar Receptors, Metabotropic Glutamate/drug effects Synaptic Transmission/drug effects Time Factors
Chemicals
Cations Neuroprotective Agents Receptors, Metabotropic Glutamate Cycloleucine 1-amino-1,3-dicarboxycyclopentane Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Congar P
Université René Descartes and Institut National de la Santé et de la Recherche Médicale Unité 29, 75674 Paris Cedex 14, France.
Leinekugel X
Ben-Ari Y
Crépel V
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1997-07-15
Pages
5366-79
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793810
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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