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PMID: 11416139 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split.

Molecular and cellular biology ·Vol. 21 ·No. 14 ·2001-07-00 ·Pages 4614-25

Yu X, Li P, Roeder RG, Wang Z

Abstract

A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription system and interacted specifically with the basal transcription factor (TFIIE) in HeLa nuclear extract. These observations implicate AES as a selective repressor of ligand-dependent AR-mediated transcription that acts by directly interacting with AR and by targeting the basal transcription machinery.

MeSH Terms
Androgen Receptor Antagonists Co-Repressor Proteins Epithelial Cells/cytology Gene Expression Regulation HeLa Cells Humans Male Prostate/cytology Proteins Receptors, Androgen/genetics,metabolism Repressor Proteins/genetics,metabolism Transcription Factors/genetics,physiology Transcription Factors, TFII Transcription, Genetic
Chemicals
Androgen Receptor Antagonists Co-Repressor Proteins Proteins Receptors, Androgen Repressor Proteins TLE5 protein, human Transcription Factors Transcription Factors, TFII transcription factor TFIIE
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yu X
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10021, USA.
Li P
Roeder R G
Wang Z
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.14.4614-4625.2001
Published
2001-07-00
Pages
4614-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87125
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.21.14.4614-4625.2001 PMC Full Text View journal details
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