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PMID: 11416153 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Insulin-responsive compartments containing GLUT4 in 3T3-L1 and CHO cells: regulation by amino acid concentrations.

Molecular and cellular biology ·Vol. 21 ·No. 14 ·2001-07-00 ·Pages 4785-806

Bogan JS, McKee AE, Lodish HF

Abstract

In fat and muscle, insulin stimulates glucose uptake by rapidly mobilizing the GLUT4 glucose transporter from a specialized intracellular compartment to the plasma membrane. We describe a method to quantify the relative proportion of GLUT4 at the plasma membrane, using flow cytometry to measure a ratio of fluorescence intensities corresponding to the cell surface and total amounts of a tagged GLUT4 reporter in individual living cells. Using this assay, we demonstrate that both 3T3-L1 and CHO cells contain intracellular compartments from which GLUT4 is rapidly mobilized by insulin and that the initial magnitude and kinetics of redistribution to the plasma membrane are similar in these two cell types when they are cultured identically. Targeting of GLUT4 to a highly insulin-responsive compartment in CHO cells is modulated by culture conditions. In particular, we find that amino acids regulate distribution of GLUT4 to this kinetically defined compartment through a rapamycin-sensitive pathway. Amino acids also modulate the magnitude of insulin-stimulated translocation in 3T3-L1 adipocytes. Our results indicate a novel link between glucose and amino acid metabolism.

MeSH Terms
3T3 Cells Amino Acids/metabolism Animals CHO Cells Cell Differentiation Cell Membrane/metabolism Cricetinae Culture Media Glucose Transporter Type 4 Humans Insulin/metabolism Kinetics Mice Monosaccharide Transport Proteins/metabolism Muscle Proteins
Chemicals
Amino Acids Culture Media Glucose Transporter Type 4 Insulin Monosaccharide Transport Proteins Muscle Proteins SLC2A4 protein, human Slc2a4 protein, mouse
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bogan J S
Diabetes Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
McKee A E
Lodish H F
References (113)
113 references, click to expand
  1. Roles of insulin, guanosine 5'-[gamma-thio]triphosphate and phorbol 12-myristate 13-acetate in signalling pathways of GLUT4 translocation.
    Biochem J. 1996 May 1;315 ( Pt 3):875-82 PMID: 8645171
  2. Overexpression of catalytic subunit p110alpha of phosphatidylinositol 3-kinase increases glucose transport activity with translocation of glucose transporters in 3T3-L1 adipocytes.
    J Biol Chem. 1996 Jul 19;271(29):16987-90 PMID: 8663584
  3. Insulin-stimulated glucose uptake involves the transition of glucose transporters to a caveolae-rich fraction within the plasma membrane: implications for type II diabetes.
    Mol Med. 1996 May;2(3):367-72 PMID: 8784789
  4. Dynamics of insulin-stimulated translocation of GLUT4 in single living cells visualised using green fluorescent protein.
    FEBS Lett. 1996 Sep 16;393(2-3):179-84 PMID: 8814285
  5. Insulin regulation and selective segregation with glucose transporter-4 of the membrane aminopeptidase vp165 in rat skeletal muscle cells.
    Endocrinology. 1997 Mar;138(3):1029-34 PMID: 9048605
  6. Functional studies in 3T3L1 cells support a role for SNARE proteins in insulin stimulation of GLUT4 translocation.
    Biochem J. 1997 May 15;324 ( Pt 1):217-24 PMID: 9164859
  7. The prolactin receptor and severely truncated erythropoietin receptors support differentiation of erythroid progenitors.
    J Biol Chem. 1997 May 30;272(22):14009-12 PMID: 9162017
  8. Vp165 and GLUT4 share similar vesicle pools along their trafficking pathways in rat adipose cells.
    FEBS Lett. 1997 Jun 16;409(3):461-8 PMID: 9224710
  9. Identification and characterization of two distinct intracellular GLUT4 pools in rat skeletal muscle: evidence for an endosomal and an insulin-sensitive GLUT4 compartment.
    Biochem J. 1997 Aug 1;325 ( Pt 3):727-32 PMID: 9271094
  10. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.
    Cell. 1975 May;5(1):19-27 PMID: 165899
  11. Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):285-9 PMID: 6928620
  12. Potential mechanism of insulin action on glucose transport in the isolated rat adipose cell. Apparent translocation of intracellular transport systems to the plasma membrane.
    J Biol Chem. 1980 May 25;255(10):4758-62 PMID: 6989818
  13. Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2542-5 PMID: 6771756
  14. Insulin-stimulated translocation of glucose transport systems in the isolated rat adipose cell. Time course, reversal, insulin concentration dependency, and relationship to glucose transport activity.
    J Biol Chem. 1981 May 25;256(10):4772-7 PMID: 7014557
  15. Evidence for the involvement of vicinal sulfhydryl groups in insulin-activated hexose transport by 3T3-L1 adipocytes.
    J Biol Chem. 1985 Mar 10;260(5):2646-52 PMID: 3882699
  16. Insulin-dependent phosphorylation of the insulin receptor-protein kinase and activation of glucose transport in 3T3-L1 adipocytes.
    J Biol Chem. 1986 Sep 15;261(26):12272-81 PMID: 3528154
  17. Regulation of insulin-stimulated glucose transport in the isolated rat adipocyte. cAMP-independent effects of lipolytic and antilipolytic agents.
    J Biol Chem. 1987 Jan 5;262(1):245-53 PMID: 3025204
  18. A glucose transport protein expressed predominately in insulin-responsive tissues.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2535-9 PMID: 2649883
  19. Insulin regulation of the two glucose transporters in 3T3-L1 adipocytes.
    J Biol Chem. 1990 Aug 15;265(23):13801-8 PMID: 2199443
  20. Cell surface labeling of glucose transporter isoform GLUT4 by bis-mannose photolabel. Correlation with stimulation of glucose transport in rat adipose cells by insulin and phorbol ester.
    J Biol Chem. 1990 Oct 25;265(30):18172-9 PMID: 2211693
  21. Differential sorting of two glucose transporters expressed in insulin-sensitive cells.
    Am J Physiol. 1991 Mar;260(3 Pt 1):C570-80 PMID: 2003579
  22. Immuno-localization of the insulin regulatable glucose transporter in brown adipose tissue of the rat.
    J Cell Biol. 1991 Apr;113(1):123-35 PMID: 2007617
  23. Immunoelectron microscopic demonstration of insulin-stimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4.
    Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6893-7 PMID: 1713695
  24. Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell type.
    J Cell Biol. 1991 Aug;114(4):689-99 PMID: 1651337
  25. Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat.
    Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7815-9 PMID: 1881917
  26. Determination of the rates of appearance and loss of glucose transporters at the cell surface of rat adipose cells.
    Biochem J. 1991 Aug 15;278 ( Pt 1):235-41 PMID: 1883332
  27. Isoform-specific subcellular targeting of glucose transporters in mouse fibroblasts.
    J Cell Biol. 1992 Feb;116(3):785-97 PMID: 1309819
  28. Trafficking of glucose transporters in 3T3-L1 cells. Inhibition of trafficking by phenylarsine oxide implicates a slow dissociation of transporters from trafficking proteins.
    Biochem J. 1992 Feb 1;281 ( Pt 3):809-17 PMID: 1536656
  29. Two glucose transporter isoforms are sorted differentially and are expressed in distinct cellular compartments.
    Biochem J. 1992 Feb 1;281 ( Pt 3):829-34 PMID: 1536658
  30. Regulation of insulin-stimulated GLUT4 translocation by Munc18c in 3T3L1 adipocytes.
    J Biol Chem. 1998 Dec 11;273(50):33876-83 PMID: 9837979
  31. Host control of HIV-1 parasitism in T cells by the nuclear factor of activated T cells.
    Cell. 1998 Nov 25;95(5):595-604 PMID: 9845362
  32. Regulation of translational effectors by amino acid and mammalian target of rapamycin signaling pathways. Possible involvement of autophagy in cultured hepatoma cells.
    J Biol Chem. 1999 Jan 8;274(2):1058-65 PMID: 9873051
  33. Signaling mechanisms that regulate glucose transport.
    J Biol Chem. 1999 Jan 22;274(4):1865-8 PMID: 9890935
  34. Molecular basis of insulin-stimulated GLUT4 vesicle trafficking. Location! Location! Location!
    J Biol Chem. 1999 Jan 29;274(5):2593-6 PMID: 9915783
  35. GLUT4 gene regulation and manipulation.
    J Biol Chem. 1999 Feb 5;274(6):3253-6 PMID: 9920861
  36. Analysis of amino and carboxy terminal GLUT-4 targeting motifs in 3T3-L1 adipocytes using an endosomal ablation technique.
    Biochemistry. 1999 Feb 2;38(5):1456-62 PMID: 9931010
  37. The formation of an insulin-responsive vesicular cargo compartment is an early event in 3T3-L1 adipocyte differentiation.
    Mol Biol Cell. 1999 May;10(5):1581-94 PMID: 10233164
  38. Regulation of ob gene expression and leptin secretion by insulin and dexamethasone in rat adipocytes.
    Diabetes. 1999 Feb;48(2):272-8 PMID: 10334301
  39. Rapamycin-sensitive phosphorylation of PKC on a carboxy-terminal site by an atypical PKC complex.
    Curr Biol. 1999 May 20;9(10):522-9 PMID: 10339425
  40. Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4.
    J Cell Biol. 1999 Aug 9;146(3):609-20 PMID: 10444069
  41. Identification of wortmannin-sensitive targets in 3T3-L1 adipocytes. DissociationoOf insulin-stimulated glucose uptake and glut4 translocation.
    J Biol Chem. 1999 Aug 27;274(35):24677-84 PMID: 10455135
  42. Insulin regulation of protein traffic in rat adipose cells.
    J Biol Chem. 1999 Sep 3;274(36):25210-7 PMID: 10464241
  43. Expression of human (beta)3-adrenergic receptor induces adipocyte-like features in CHO/K1 fibroblasts.
    J Cell Sci. 1999 Nov;112 ( Pt 21):3791-7 PMID: 10523514
  44. A phosphatidylinositol 3-Kinase/p70 ribosomal S6 protein kinase pathway is required for the regulation by insulin of the p85alpha regulatory subunit of phosphatidylinositol 3-kinase gene expression in human muscle cells.
    J Biol Chem. 1999 Nov 26;274(48):34005-10 PMID: 10567366
  45. Mammalian TOR controls one of two kinase pathways acting upon nPKCdelta and nPKCepsilon.
    J Biol Chem. 1999 Dec 3;274(49):34758-64 PMID: 10574945
  46. Characterization of insulin-responsive GLUT4 storage vesicles isolated from 3T3-L1 adipocytes.
    Mol Cell Biol. 2000 Jan;20(1):416-27 PMID: 10594043
  47. Separation and partial characterization of three distinct intracellular GLUT4 compartments in rat adipocytes. Subcellular fractionation without homogenization.
    J Biol Chem. 1999 Dec 31;274(53):37755-62 PMID: 10608836
  48. Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins.
    Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14866-70 PMID: 10611304
  49. Insulin-responsive aminopeptidase trafficking in 3T3-L1 adipocytes.
    J Biol Chem. 2000 Jan 28;275(4):2560-7 PMID: 10644714
  50. Overexpression of the glucose transporter GLUT4 in adipose cells interferes with insulin-stimulated translocation.
    FEBS Lett. 1999 Oct 29;460(2):338-42 PMID: 10544260
  51. Insulin recruits GLUT4 from specialized VAMP2-carrying vesicles as well as from the dynamic endosomal/trans-Golgi network in rat adipocytes.
    Mol Biol Cell. 2000 Dec;11(12):4079-91 PMID: 11102509
  52. Serine/threonine phosphorylation of IRS-1 triggers its degradation: possible regulation by tyrosine phosphorylation.
    Diabetes. 2001 Jan;50(1):24-31 PMID: 11147790
  53. Frap-dependent serine phosphorylation of IRS-1 inhibits IRS-1 tyrosine phosphorylation.
    Biochem Biophys Res Commun. 2001 Jan 26;280(3):776-81 PMID: 11162588
  54. Insulin action on GLUT4 traffic visualized in single 3T3-l1 adipocytes by using ultra-fast microscopy.
    Mol Biol Cell. 2001 Jan;12(1):129-41 PMID: 11160828
  55. GLUT4--at the cross roads between membrane trafficking and signal transduction.
    Traffic. 2001 Jan;2(1):2-11 PMID: 11208163
  56. New perspectives into the molecular pathogenesis and treatment of type 2 diabetes.
    Cell. 2001 Feb 23;104(4):517-29 PMID: 11239409
  57. Development of an intracellular pool of glucose transporters in 3T3-L1 cells.
    J Biol Chem. 1992 May 25;267(15):10393-9 PMID: 1587825
  58. Translocation of the glucose transporter (GLUT4) to the cell surface in permeabilized 3T3-L1 adipocytes: effects of ATP insulin, and GTP gamma S and localization of GLUT4 to clathrin lattices.
    J Cell Biol. 1992 Jun;117(6):1181-96 PMID: 1607382
  59. Subcellular distribution and activity of glucose transporter isoforms GLUT1 and GLUT4 transiently expressed in COS-7 cells.
    Biochim Biophys Acta. 1992 Jul 15;1131(3):245-52 PMID: 1627641
  60. Effects of insulin on steady state kinetics of GLUT4 subcellular distribution in rat adipocytes. Evidence of constitutive GLUT4 recycling.
    J Biol Chem. 1992 Sep 5;267(25):17710-5 PMID: 1517217
  61. Domains responsible for the differential targeting of glucose transporter isoforms.
    J Biol Chem. 1992 Sep 25;267(27):19636-41 PMID: 1527083
  62. Efficient retroviral-mediated gene transfer into human B lymphoblastoid cells expressing mouse ecotropic viral receptor.
    Nucleic Acids Res. 1992 Oct 11;20(19):5234 PMID: 1408844
  63. Cell surface accessibility of GLUT4 glucose transporters in insulin-stimulated rat adipose cells. Modulation by isoprenaline and adenosine.
    Biochem J. 1992 Nov 15;288 ( Pt 1):325-30 PMID: 1445278
  64. Dissociation of pp70 ribosomal protein S6 kinase from insulin-stimulated glucose transport in 3T3-L1 adipocytes.
    J Biol Chem. 1993 Feb 5;268(4):3005-8 PMID: 7679106
  65. Comparison of GLUT4 and GLUT1 subcellular trafficking in basal and insulin-stimulated 3T3-L1 cells.
    J Biol Chem. 1993 Mar 5;268(7):4600-3 PMID: 8444835
  66. Direct demonstration of insulin-induced GLUT4 translocation to the surface of intact cells by insertion of a c-myc epitope into an exofacial GLUT4 domain.
    J Biol Chem. 1993 Jul 5;268(19):14523-6 PMID: 7686158
  67. Targeting of the "insulin-responsive" glucose transporter (GLUT4) to the regulated secretory pathway in PC12 cells.
    J Cell Biol. 1993 Aug;122(3):579-88 PMID: 8335686
  68. Use of bismannose photolabel to elucidate insulin-regulated GLUT4 subcellular trafficking kinetics in rat adipose cells. Evidence that exocytosis is a critical site of hormone action.
    J Biol Chem. 1993 Aug 25;268(24):17820-9 PMID: 8349666
  69. Exofacial epitope-tagged glucose transporter chimeras reveal COOH-terminal sequences governing cellular localization.
    J Cell Biol. 1993 Oct;123(1):127-35 PMID: 8408193
  70. Identification of the carboxy terminus as important for the isoform-specific subcellular targeting of glucose transporter proteins.
    J Cell Biol. 1993 Oct;123(1):137-47 PMID: 7691826
  71. Establishment and characterization of differentiated, nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha.
    Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):674-8 PMID: 7904757
  72. Tyrosine kinase-deficient mutant human insulin receptors (Met1153-->Ile) overexpressed in transfected rat adipose cells fail to mediate translocation of epitope-tagged GLUT4.
    Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5587-91 PMID: 8202531
  73. Phosphatidylinositol 3-kinase activation is required for insulin stimulation of pp70 S6 kinase, DNA synthesis, and glucose transporter translocation.
    Mol Cell Biol. 1994 Jul;14(7):4902-11 PMID: 8007986
  74. Insulin-stimulated GLUT4 glucose transporter recycling. A problem in membrane protein subcellular trafficking through multiple pools.
    J Biol Chem. 1994 Jul 1;269(26):17516-24 PMID: 8021259
  75. Induction of caveolin during adipogenesis and association of GLUT4 with caveolin-rich vesicles.
    J Cell Biol. 1994 Dec;127(5):1233-43 PMID: 7962086
  76. A distinct class of intracellular storage vesicles, identified by expression of the glucose transporter GLUT4.
    Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12750-4 PMID: 7809115
  77. Expression and compartmentalization of caveolin in adipose cells: coordinate regulation with and structural segregation from GLUT4.
    J Cell Biol. 1995 May;129(4):999-1006 PMID: 7744970
  78. Possible domains responsible for intracellular targeting and insulin-dependent translocation of glucose transporter type 4.
    Biochem J. 1995 Aug 1;309 ( Pt 3):813-23 PMID: 7543750
  79. Molecular regulation of GLUT-4 targeting in 3T3-L1 adipocytes.
    J Cell Biol. 1995 Sep;130(5):1081-91 PMID: 7544796
  80. A novel serum protein similar to C1q, produced exclusively in adipocytes.
    J Biol Chem. 1995 Nov 10;270(45):26746-9 PMID: 7592907
  81. Peroxisome proliferator activated receptor gamma, CCAAT/enhancer-binding protein alpha, and cell cycle status regulate the commitment to adipocyte differentiation.
    J Biol Chem. 1997 Aug 22;272(34):21473-8 PMID: 9261165
  82. Immunocytochemical evidence that GLUT4 resides in a specialized translocation post-endosomal VAMP2-positive compartment in rat adipose cells in the absence of insulin.
    J Histochem Cytochem. 1997 Aug;45(8):1083-96 PMID: 9267469
  83. Trafficking kinetics of the insulin-regulated membrane aminopeptidase in 3T3-L1 adipocytes.
    Biochem Biophys Res Commun. 1997 Oct 9;239(1):247-51 PMID: 9345303
  84. GLUT4 vesicle dynamics in living 3T3 L1 adipocytes visualized with green-fluorescent protein.
    Biochem J. 1997 Nov 1;327 ( Pt 3):637-42 PMID: 9581537
  85. Moving GLUT4: the biogenesis and trafficking of GLUT4 storage vesicles.
    Diabetes. 1997 Nov;46(11):1667-77 PMID: 9356011
  86. The glucose transporter GLUT4 and the aminopeptidase vp165 colocalise in tubulo-vesicular elements in adipocytes and cardiomyocytes.
    J Cell Sci. 1997 Sep;110 ( Pt 18):2281-91 PMID: 9378777
  87. Morphological effects of wortmannin on the endosomal system and GLUT4-containing compartments in rat adipose cells.
    J Cell Sci. 1997 Nov;110 ( Pt 22):2795-806 PMID: 9427288
  88. GLUT4 and transferrin receptor are differentially sorted along the endocytic pathway in CHO cells.
    J Cell Biol. 1998 Feb 9;140(3):565-75 PMID: 9456317
  89. Possible role for gp160 in constitutive but not insulin-stimulated GLUT4 trafficking: dissociation of gp160 and GLUT4 localization.
    Biochem J. 1998 Feb 15;330 ( Pt 1):405-11 PMID: 9461537
  90. RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1.
    Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1432-7 PMID: 9465032
  91. Increased intracellular sequestration of the insulin-regulated aminopeptidase upon differentiation of 3T3-L1 cells.
    Biochem J. 1998 Mar 1;330 ( Pt 2):1003-8 PMID: 9480922
  92. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor.
    Curr Biol. 1998 Mar 12;8(6):335-8 PMID: 9512423
  93. Bidirectional modulation of insulin action by amino acids.
    J Clin Invest. 1998 Apr 1;101(7):1519-29 PMID: 9525995
  94. Membrane-permeant esters of phosphatidylinositol 3,4,5-trisphosphate.
    J Biol Chem. 1998 May 1;273(18):11017-24 PMID: 9556583
  95. Multiple endosomal recycling pathways in rat adipose cells.
    Biochem J. 1998 May 1;331 ( Pt 3):829-35 PMID: 9560311
  96. GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay.
    FEBS Lett. 1998 May 8;427(2):193-7 PMID: 9607310
  97. Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism.
    J Biol Chem. 1998 Jun 5;273(23):14484-94 PMID: 9603962
  98. Evidence of insulin-stimulated phosphorylation and activation of the mammalian target of rapamycin mediated by a protein kinase B signaling pathway.
    Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7772-7 PMID: 9636226
  99. Identification of an insulin-responsive, slow endocytic recycling mechanism in Chinese hamster ovary cells.
    J Biol Chem. 1998 Jul 10;273(28):17968-77 PMID: 9651404
  100. Expression of a dominant interfering dynamin mutant in 3T3L1 adipocytes inhibits GLUT4 endocytosis without affecting insulin signaling.
    J Biol Chem. 1998 Sep 25;273(39):25450-7 PMID: 9738014
  101. Characterization of the insulin-regulated endocytic recycling mechanism in 3T3-L1 adipocytes using a novel reporter molecule.
    J Biol Chem. 2000 Feb 18;275(7):4787-95 PMID: 10671512
  102. A rapamycin-sensitive pathway down-regulates insulin signaling via phosphorylation and proteasomal degradation of insulin receptor substrate-1.
    Mol Endocrinol. 2000 Jun;14(6):783-94 PMID: 10847581
  103. Amino acids regulate skeletal muscle PHAS-I and p70 S6-kinase phosphorylation independently of insulin.
    Am J Physiol Endocrinol Metab. 2000 Aug;279(2):E301-6 PMID: 10913029
  104. Modulation of GLUT4 and GLUT1 recycling by insulin in rat adipocytes: kinetic analysis based on the involvement of multiple intracellular compartments.
    Biochemistry. 2000 Aug 8;39(31):9358-66 PMID: 10924130
  105. Effects of insulin on intracellular GLUT4 vesicles in adipocytes: evidence for a secretory mode of regulation.
    J Cell Sci. 2000 Oct;113 Pt 19:3427-38 PMID: 10984434
  106. CAP defines a second signalling pathway required for insulin-stimulated glucose transport.
    Nature. 2000 Sep 14;407(6801):202-7 PMID: 11001060
  107. TOR, a central controller of cell growth.
    Cell. 2000 Oct 13;103(2):253-62 PMID: 11057898
  108. Demonstration of insulin-responsive trafficking of GLUT4 and vpTR in fibroblasts.
    J Cell Sci. 2000 Nov;113 ( Pt 22):4065-76 PMID: 11058093
  109. Kinetic analysis of glucose transporter trafficking in fibroblasts and adipocytes.
    Biochemistry. 1995 Nov 28;34(47):15523-31 PMID: 7492554
  110. Applications of retrovirus-mediated expression cloning.
    Exp Hematol. 1996 Feb;24(2):324-9 PMID: 8641361
  111. Compartment ablation analysis of the insulin-responsive glucose transporter (GLUT4) in 3T3-L1 adipocytes.
    Biochem J. 1996 Apr 15;315 ( Pt 2):487-95 PMID: 8615819
  112. Subcellular trafficking kinetics of GLU4 mutated at the N- and C-terminal.
    Biochem J. 1996 Apr 1;315 ( Pt 1):153-9 PMID: 8670101
  113. Characterization of the insulin-regulated membrane aminopeptidase in 3T3-L1 adipocytes.
    J Biol Chem. 1996 Feb 9;271(6):3328-32 PMID: 8621739
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.14.4785-4806.2001
Published
2001-07-00
Pages
4785-806
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87167
Subset
IM
Grants
NIDDK NIH HHS · R37 DK047618 · United States
NIDDK NIH HHS · K11 DK02371 · United States
NIDDK NIH HHS · R37 DK47618 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.14.4785-4806.2001 PMC Full Text View journal details
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