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PMID: 2649883 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A glucose transport protein expressed predominately in insulin-responsive tissues.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 8 ·1989-04-00 ·Pages 2535-9

Charron MJ, Brosius FC, Alper SL, Lodish HF

Abstract

Using low-stringency hybridization to the rat brain glucose transporter (GT), a 2489-base-pair cDNA clone was isolated from a rat soleus lambda gt10 cDNA library. It encodes a 509-amino acid protein whose sequence and predicted membrane structure is very similar to those of the rat brain and liver GTs. The muscle GT-like protein is 65% identical in amino acid sequence to the rat brain GT and 52% identical to the rat liver GT; the major differences are in the NH2- and COOH-terminal hydrophilic segments. This GT-like mRNA is expressed predominately in tissues where glucose transport is sensitive to insulin, including striated muscle, cardiac muscle, and adipose tissue; low-level expression is also detected in smooth muscle and kidney mRNA. This GT-like cDNA is the fourth member of the mammalian GT-related gene family identified to date. We propose that it encodes an insulin-sensitive GT.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Insulin/physiology Molecular Sequence Data Monosaccharide Transport Proteins/genetics Multigene Family Muscles/physiology Rats Tissue Distribution
Chemicals
Insulin Monosaccharide Transport Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Charron M J
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Brosius F C
Alper S L
Lodish H F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.8.2535
Published
1989-04-00
Pages
2535-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286951
Subset
IM
Grants
NIADDK NIH HHS · AM01506 · United States
NHLBI NIH HHS · HL41484 · United States
Databases
GENBANK
J04524
External Links
PubMed source DOI: 10.1073/pnas.86.8.2535 PMC Full Text View journal details
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