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PMID: 11424189 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons.

Glia ·Vol. 35 ·No. 1 ·2001-07-00 ·Pages 26-34

Sasaki M, Honmou O, Akiyama Y, Uede T, Hashi K, Kocsis JD

Abstract

The potential of bone marrow cells to differentiate into myelin-forming cells and to repair the demyelinated rat spinal cord in vivo was studied using cell transplantation techniques. The dorsal funiculus of the spinal cord was demyelinated by x-irradiation treatment, followed by microinjection of ethidium bromide. Suspensions of a bone marrow cell fraction acutely isolated from femoral bones in LacZ transgenic mice were prepared by centrifugation on a density gradient (Ficoll-Paque) to remove erythrocytes, platelets, and debris. The isolated cell fraction contained hematopoietic and nonhematopoietic stem and precursor cells and lymphocytes. The cells were transplanted into the demyelinated dorsal column lesions of immunosuppressed rats. An intense blue beta-galactosidase reaction was observed in the transplantation zone. The genetically labeled bone marrow cells remyelinated the spinal cord with predominately a peripheral pattern of myelination reminiscent of Schwann cell myelination. Transplantation of CD34(+) hematopoietic stem cells survived in the lesion, but did not form myelin. These results indicate that bone marrow cells can differentiate in vivo into myelin-forming cells and repair demyelinated CNS.

MeSH Terms
Animals Animals, Newborn Bone Marrow Transplantation/methods Cells, Cultured/cytology,metabolism,transplantation Ethidium/pharmacology Glial Fibrillary Acidic Protein/metabolism Immunohistochemistry Mice Mice, Transgenic Myeloid Progenitor Cells/cytology,metabolism,transplantation Nerve Fibers, Myelinated/pathology,ultrastructure Nerve Regeneration/physiology Neuroglia/drug effects,metabolism,ultrastructure Rats Rats, Wistar Recovery of Function/physiology Spinal Cord/growth & development,surgery,ultrastructure Spinal Cord Injuries/pathology,physiopathology,surgery beta-Galactosidase
Chemicals
Glial Fibrillary Acidic Protein beta-Galactosidase Ethidium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sasaki M
Department of Neurosurgery, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, Japan.
Honmou O
Akiyama Y
Uede T
Hashi K
Kocsis J D
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Article Info
Journal
Glia
Abbr.
Glia
ISSN
0894-1491
DOI
10.1002/glia.1067
Published
2001-07-00
Pages
26-34
Language
English
Region
United States
NLM ID
8806785
PMCID
PMC2605363
Subset
IM
Grants
NINDS NIH HHS · F05 NS010174 · United States
NINDS NIH HHS · P50 NS010174-290027 · United States
NINDS NIH HHS · NS10174 · United States
External Links
PubMed source DOI: 10.1002/glia.1067 PMC Full Text View journal details
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