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PMID: 1142477 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of DNA polymerase gamma from an adenovirus 2 DNA replication complex.

Journal of virology ·Vol. 15 ·No. 6 ·1975-06-00 ·Pages 1507-10

Ito K, Arens M, Green M

Abstract

The major DNA polymerase in a nuclear membrane complex that is capable of synthesizing viral DNA sequences in vitro has been purified about 900-fold from adenovirus 2-infected KB cells. The enzyme was characterized as belonging to the class of mammalian DNA polymerases (DNA polymerase gamma) that can utilize poly(A) with oligo(dT) as template primer.

MeSH Terms
Adenoviridae/metabolism Carcinoma Cell Fractionation Cell Line Cell Nucleus/enzymology Cell-Free System Centrifugation, Density Gradient Chromatography Chromatography, DEAE-Cellulose DNA Nucleotidyltransferases/isolation & purification,metabolism DNA Replication DNA, Viral/biosynthesis Humans Manganese/pharmacology Molecular Weight Mouth Neoplasms Polyethylene Glycols Polynucleotides/metabolism Templates, Genetic
Chemicals
DNA, Viral Polynucleotides Polyethylene Glycols Manganese DNA Nucleotidyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ito K
Arens M
Green M
References (18)
18 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.6.1507-1510.1975
Published
1975-06-00
Pages
1507-10
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354619
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.6.1507-1510.1975 PMC Full Text View journal details
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