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PMID: 11427575 Published · ppublish English Journal Article

Detection of RTX toxin gene in Vibrio cholerae by PCR.

Journal of clinical microbiology ·Vol. 39 ·No. 7 ·2001-07-00 ·Pages 2594-7

Chow KH, Ng TK, Yuen KY, Yam WC

Abstract

A PCR that amplifies a recently discovered Vibrio cholerae RTX (repeat in toxin) toxin gene was developed. Among 166 clinical and environmental isolates of V. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both PCR and HEp-2 cell cytotoxicity assay. Standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. This is the first rapid genotyping method for differentiation of V. cholerae O1 classical biotype strains from El Tor biotype strains as well as strains of other non-O1 serogroups including serogroup O139. The PCR assay that was developed also specifically detects RTX toxin genes in V. cholerae, as clinical isolates of Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Aeromonas species, and Plesiomonas species were all negative by the RTX toxin-specific PCR as well as the HEp-2 cytotoxicity assay. These findings highlight the characteristics of the RTX toxins in V. cholerae. Their role in the pathogenicity of the bacterium requires further investigation.

MeSH Terms
Acyltransferases/genetics,metabolism,toxicity Bacterial Proteins Bacterial Toxins/genetics,metabolism,toxicity Cell Line Cholera/microbiology Environmental Microbiology Genes, Bacterial Humans Polymerase Chain Reaction/methods Vibrio cholerae/genetics,metabolism
Chemicals
Bacterial Proteins Bacterial Toxins RtxA protein, Vibrio cholerae Acyltransferases RtxC protein, Vibrio cholerae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chow K H
Department of Microbiology, The University of Hong Kong, China.
Ng T K
Yuen K Y
Yam W C
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.39.7.2594-2597.2001
Published
2001-07-00
Pages
2594-7
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC88191
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.39.7.2594-2597.2001 PMC Full Text View journal details
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