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PMID: 11432846 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

VIP1, an Arabidopsis protein that interacts with Agrobacterium VirE2, is involved in VirE2 nuclear import and Agrobacterium infectivity.

The EMBO journal ·Vol. 20 ·No. 13 ·2001-07-02 ·Pages 3596-607

Tzfira T, Vaidya M, Citovsky V

Abstract

T-DNA nuclear import is a central event in genetic transformation of plant cells by Agrobacterium. This event is thought to be mediated by two bacterial proteins, VirD2 and VirE2, which are associated with the transported T-DNA molecule. While VirD2 is imported into the nuclei of plant, animal and yeast cells, nuclear uptake of VirE2 occurs most efficiently in plant cells. To understand better the mechanism of VirE2 action, a cellular interactor of VirE2 was identified and its encoding gene cloned from Arabidopsis. The identified plant protein, designated VIP1, specifically bound VirE2 and allowed its nuclear import in non-plant systems. In plants, VIP1 was required for VirE2 nuclear import and Agrobacterium tumorigenicity, participating in early stages of T-DNA expression.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/genetics,microbiology,physiology Arabidopsis Proteins Bacterial Proteins COS Cells Cell Nucleus/physiology Chlorocebus aethiops DNA, Bacterial/genetics DNA-Binding Proteins/metabolism Genes, Reporter Glucuronidase/genetics Ion Channels/metabolism Membrane Proteins/metabolism Molecular Sequence Data Plant Proteins/chemistry,genetics,metabolism Plant Tumors Plants, Genetically Modified Plants, Toxic Protein Binding Protein Transport Recombinant Fusion Proteins/metabolism Reverse Transcriptase Polymerase Chain Reaction Rhizobium/pathogenicity,physiology Saccharomyces cerevisiae/physiology Sequence Alignment Sequence Homology, Amino Acid Tobacco/microbiology,physiology Transfection
Chemicals
Arabidopsis Proteins Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Ion Channels Membrane Proteins Plant Proteins Recombinant Fusion Proteins T-DNA VIP1 protein, Arabidopsis virE2 protein, Agrobacterium Glucuronidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tzfira T
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA.
Vaidya M
Citovsky V
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.13.3596
Published
2001-07-02
Pages
3596-607
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125502
Subset
IM
Databases
GENBANK
AC009526, AF225983
External Links
PubMed source DOI: 10.1093/emboj/20.13.3596 PMC Full Text View journal details
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