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PMID: 11481431 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 17 ·2001-08-14 ·Pages 9889-94

Finan TM, Weidner S, Wong K, Buhrmester J, Chain P, Vorhölter FJ, Hernandez-Lucas I, Becker A, Cowie A, Gouzy J, Golding B, Pühler A

Abstract

Analysis of the 1,683,333-nt sequence of the pSymB megaplasmid from the symbiotic N(2)-fixing bacterium Sinorhizobium meliloti revealed that the replicon has a high gene density with a total of 1,570 protein-coding regions, with few insertion elements and regions duplicated elsewhere in the genome. The only copies of an essential arg-tRNA gene and the minCDE genes are located on pSymB. Almost 20% of the pSymB sequence carries genes encoding solute uptake systems, most of which were of the ATP-binding cassette family. Many previously unsuspected genes involved in polysaccharide biosynthesis were identified and these, together with the two known distinct exopolysaccharide synthesis gene clusters, show that 14% of the pSymB sequence is dedicated to polysaccharide synthesis. Other recognizable gene clusters include many involved in catabolic activities such as protocatechuate utilization and phosphonate degradation. The functions of these genes are consistent with the notion that pSymB plays a major role in the saprophytic competence of the bacteria in the soil environment.

MeSH Terms
Bacterial Proteins/genetics,physiology Carbohydrate Metabolism Carrier Proteins/genetics Chaperonins/genetics DNA, Bacterial/genetics Drug Resistance, Microbial/genetics Enzymes/genetics Gene Expression Regulation, Bacterial Gene Library Genes, Bacterial Lipopolysaccharides/biosynthesis Molecular Sequence Data Nitrogen Fixation/genetics Plasmids/genetics RNA, Transfer, Arg/genetics Replication Origin/genetics Replicon/genetics Sequence Analysis, DNA Sinorhizobium meliloti/genetics Species Specificity Transcription, Genetic/genetics
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial Enzymes Lipopolysaccharides RNA, Transfer, Arg Chaperonins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Finan T M
Department of Biology, McMaster University, Hamilton, ON, Canada. [email protected]
Weidner S
Wong K
Buhrmester J
Chain P
Vorhölter F J
Hernandez-Lucas I
Becker A
Cowie A
Gouzy J
Golding B
Pühler A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.161294698
Email
[email protected]
Published
2001-08-14
Epub
2001-00-31
Pages
9889-94
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55548
Subset
IM
Databases
GENBANK
AL591985
External Links
PubMed source DOI: 10.1073/pnas.161294698 PMC Full Text View journal details
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