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PMID: 11487700 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

G2/M-phase-specific transcription during the plant cell cycle is mediated by c-Myb-like transcription factors.

The Plant cell ·Vol. 13 ·No. 8 ·2001-08-00 ·Pages 1891-905

Ito M, Araki S, Matsunaga S, Itoh T, Nishihama R, Machida Y, Doonan JH, Watanabe A

Abstract

Plant B-type cyclin genes are expressed specifically in late G2- and M-phases during the cell cycle. Their promoters contain a common cis-acting element, called the MSA (M-specific activator) element, that is necessary and sufficient for periodic promoter activation. This motif also is present in the tobacco kinesin-like protein gene NACK1, which is expressed with timing similar to that of B-type cyclin genes. In this study, we show that G2/M-phase-specific activation of the NACK1 promoter also is regulated by the MSA element, suggesting that a defined set of G2/M-phase-specific genes are coregulated by an MSA-mediated mechanism. In a search for MSA binding factors by yeast one-hybrid screening, we identified three different Myb-like proteins that interact specifically with the MSA sequence. Unlike the majority of plant Myb-like proteins, these Myb proteins, NtmybA1, NtmybA2, and NtmybB, have three imperfect repeats in the DNA binding domain, as in animal c-Myb proteins. During the cell cycle, the level of NtmybB mRNA did not change significantly, whereas the levels of NtmybA1 and A2 mRNAs fluctuated and peaked at M-phase, when B-type cyclin genes were maximally induced. In transient expression assays, NtmybA1 and A2 activated the MSA-containing promoters, whereas NtmybB repressed them. Furthermore, expression of NtmybB repressed the transcriptional activation mediated by NtmybA2. Our data show that a group of plant Myb proteins that are structurally similar to animal c-Myb proteins have unexpected roles in G2/M-phase by modulating the expression of B-type cyclin genes and may regulate a suite of coexpressed genes.

MeSH Terms
Base Sequence DNA, Complementary G2 Phase In Situ Hybridization Mitosis Molecular Sequence Data Plants, Toxic Promoter Regions, Genetic Proto-Oncogene Proteins c-myb/genetics,physiology Tobacco/cytology Transcription Factors/genetics,physiology Transcription, Genetic/physiology
Chemicals
DNA, Complementary Proto-Oncogene Proteins c-myb Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ito M
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan. [email protected]
Araki S
Matsunaga S
Itoh T
Nishihama R
Machida Y
Doonan J H
Watanabe A
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010102
Email
[email protected]
Published
2001-08-00
Pages
1891-905
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139135
Subset
IM
Databases
GENBANK
AB056122, AB056123, AB056124, AF176005, AF214117, AF218054, J02390, M15024, P10290, P20024, P20025, P20026, S44926, T48253, T48510, X05939, X13293, X13294, Z13996, Z13997, Z13998
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1105/tpc.010102 PMC Full Text View journal details
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