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PMID: 11607492 Published · ppublish English Journal Article

Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 17 ·1994-08-16 ·Pages 8000-4

Tinland B, Hohn B, Puchta H

Abstract

Transferred DNA (T-DNA) is transferred as a single-stranded derivative from Agrobacterium to the plant cell nucleus. This conclusion is drawn from experiments exploiting the different properties of single- and double-stranded DNA to perform extrachromosomal homologous recombination in plant cells. After transfer from Agrobacterium to plant cells, T-DNA molecules recombined much more efficiently if the homologous sequences were of opposite polarity than if they were of the same polarity. This observation reflects the properties of single-stranded DNA; single-stranded DNA molecules of opposite polarity can anneal directly, whereas single-stranded DNA molecules of the same polarity first have to become double stranded to anneal. Judging from the relative amounts of single- to double-stranded T-DNA derivatives undergoing recombination, we infer that the T-DNA derivatives enter the plant nucleus in their single-stranded form.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tinland B
Friedrich Miescher-Institute, Basel, Switzerland.
Hohn B
Puchta H
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49 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.17.8000
Published
1994-08-16
Pages
8000-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44532
External Links
PubMed source DOI: 10.1073/pnas.91.17.8000 PMC Full Text View journal details
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