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PMID: 3403506 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of the virD2 protein with the 5' end of T strands in Agrobacterium tumefaciens.

Journal of bacteriology ·Vol. 170 ·No. 8 ·1988-08-00 ·Pages 3367-74

Young C, Nester EW

Abstract

The soil bacterium Agrobacterium tumefaciens can incite tumors in many dicotyledonous plants by transferring a portion (T-DNA) of its Ti plasmid into susceptible plant cells. The T-DNA is flanked by border sequences that serve as recognition sites for specific cleavage by an endonuclease that comprises two virD-encoded proteins (VirD1 and VirD2). After cleavage, both double-stranded, nicked T-DNA molecules and single-stranded T-DNA molecules (T strands) were present. We have determined that a protein is tightly associated with, and probably covalently attached to, the 5' end of the T strands. Analysis of deletion derivatives in Escherichia coli, immunoprecipitation, and a procedure combining immunoblot and nucleic acid hybridization data identified this protein as the gene product of virD2.

MeSH Terms
Bacterial Proteins/genetics,metabolism DNA, Bacterial/genetics,metabolism DNA, Single-Stranded/genetics Immunoassay Nucleic Acid Hybridization Plant Tumors/microbiology Plasmids Rhizobium/genetics
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Single-Stranded
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Young C
Department of Microbiology, University of Washington, Seattle 98195.
Nester E W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.8.3367-3374.1988
Published
1988-08-00
Pages
3367-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211303
Subset
IM
Grants
NIGMS NIH HHS · 5-F32-GM11082 · United States
NIGMS NIH HHS · 5-RO1-GM32618-14 · United States
External Links
PubMed source DOI: 10.1128/jb.170.8.3367-3374.1988 PMC Full Text View journal details
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