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PMID: 116191 Published · ppublish English Journal Article

Immunological purification and partial characterization of variant-specific surface antigen messenger RNA of Trypanosoma brucei brucei.

Nucleic acids research ·Vol. 7 ·No. 3 ·1979-10-10 ·Pages 595-609

Lheureux M, Lheureux M, Vervoort T, Van Meirvenne N, Steinert M

Abstract

Polyadenylated RNA isolated from total polyribosomes of two variable antigen types (VATs) of T. brucei brucei were shown to program the synthesis, in mRNA-dependant reticulocyte lysates, of a wide variety of polypeptides. After immunoprecipitation of these cell-free products with an homologous antiserum raised against purified variant-specific surface antigen (VSSA), a major electrophoretic band was apparent on fluorography. It was confirmed that this band corresponds to the variable antigen since only an excess of purified homologous antigen will provoke competition. The apparent molecular weight of the in vitro synthesized antigen is about 63,000 daltons. The VSSA mRNA has been found in membrane-bound polyribosomes and a 15 fold immunological purification of this mRNA has been obtained, using partially purified anti-VSSA IgG in conjunction with inactivated Staphylococcus aureus.

MeSH Terms
Animals Antigens, Surface Immunodiffusion Poly A/isolation & purification,metabolism Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/isolation & purification,metabolism Trypanosoma brucei brucei/metabolism
Chemicals
Antigens, Surface RNA, Messenger Poly A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lheureux M
Lheureux M
Vervoort T
Van Meirvenne N
Steinert M
References (29)
29 references, click to expand
  1. Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei.
    Parasitology. 1975 Dec;71(3):393-417 PMID: 645
  2. Antigenic variation in trypanosomes.
    Proc R Soc Lond B Biol Sci. 1978 Jun 5;202(1146):55-72 PMID: 27816
  3. Bovine leukemia virus: an exogenous RNA oncogenic virus.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1014-8 PMID: 57616
  4. [Trypanosoma equiperdum: antigenic variations in experimental trypanosomiasis of rabbits].
    Exp Parasitol. 1977 Jun;42(1):6-13 PMID: 67961
  5. Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3230-4 PMID: 198776
  6. Purification and properties of rabbit uterus preuteroglobin mRNA.
    Nucleic Acids Res. 1977 Nov;4(11):4023-36 PMID: 593899
  7. Antigenic variation in trypanosomes.
    Nature. 1978 Jun 22;273(5664):613-7 PMID: 661969
  8. A characterization of mRNA activites and their sequence complexities in Trypanosoma brucei: partial purification and properties of the VSSA mRNA.
    Nucleic Acids Res. 1978 Sep;5(9):3171-82 PMID: 704349
  9. Differential translation of turnip yellow mosaic virus mRNAs in vitro.
    Biochem Biophys Res Commun. 1978 Oct 30;84(4):831-9 PMID: 728152
  10. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  11. Translation in a reticulocyte cell-free system of RNA isolated from blood and culture forms of Trypanosoma brucei.
    Parasitology. 1977 Oct;75(2):133-41 PMID: 927883
  12. Monospecific immunoprecipitation of murine leukemia virus polyribosomes: identification of p30 protein-specific messenger RNA.
    Cell. 1976 Dec;9(4 Pt 1):579-88 PMID: 1009577
  13. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  14. Antigenic variation in syringe passaged populations of Trypanosoma (Trypanozoon) brucei. 1. Rationalization of the experimental approach.
    Ann Soc Belg Med Trop. 1975;55(1):1-23 PMID: 1231658
  15. Identification and isolation of ovalbumin-synthesizing polysomes. I. Specific binding of 125 I-anti-ovalbumin to polysomes.
    J Biol Chem. 1972 Apr 25;247(8):2316-21 PMID: 4111967
  16. On the ultrastructure of Trypanosoma (Trypanozoon) brucei in the course of its life cycle and some related aspects.
    Acta Trop. 1973;30(1):64-168 PMID: 4144959
  17. Further studies on the translation of globin mRNA and encephalomyocarditis virus RNA in a cell-free system from Krebs II ascites cells.
    Biochim Biophys Acta. 1972 Jun 22;272(1):108-18 PMID: 4339672
  18. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799-803 PMID: 4515941
  19. Translational control of globin synthesis by haemin in Xenopus oocytes.
    Nat New Biol. 1973 Nov 28;246(152):99-102 PMID: 4518941
  20. Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages.
    J Mol Biol. 1973 Nov 5;80(3):539-51 PMID: 4796973
  21. Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.
    Exp Parasitol. 1970 Dec;28(3):521-34 PMID: 4993889
  22. Polypeptides of the tail fibres of bacteriophage T4.
    J Mol Biol. 1971 Dec 28;62(3):465-77 PMID: 5136579
  23. Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation.
    J Biol Chem. 1971 Dec 10;246(23):7131-8 PMID: 5167019
  24. Synthesis of and chains of rabbit hemoglobin in a cell-free extract from Krebs II ascites cells.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2716-9 PMID: 5288249
  25. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  26. On the surface coat and flagellar adhesion in trypanosomes.
    J Cell Sci. 1969 Jul;5(1):163-93 PMID: 5353653
  27. Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture.
    J Biol Chem. 1971 Feb 10;246(3):724-37 PMID: 5542686
  28. Ribosome-bound beta-galactosidase.
    Proc Natl Acad Sci U S A. 1961 Jan 15;47:114-22 PMID: 13696108
  29. Polyribosomes from peas: an improved method for their isolation in the absence of ribonuclease inhibitors.
    Plant Physiol. 1972 Nov;50(5):581-4 PMID: 16658221
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.3.595
Published
1979-10-10
Pages
595-609
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328041
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.3.595 PMC Full Text View journal details
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