Home LiteratureArticle Details

PMID: 1168691 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Subcellular platelet factor VIII antigen and von Willebrand factor.

The Journal of experimental medicine ·Vol. 141 ·No. 5 ·1975-05-01 ·Pages 1101-13

Nachman RL, Jaffe EA

Abstract

Subcellular membrane and granule fractions derived from human platelets contain factor VIIII antigen and von Willebrand factor activity but not factor VII procoagulant activity. Circulating platelets constitute a significant reservoir of plasma factor VIII antigen, containing approximately 15% of the amount of factor VIII antigen present in platelet-poor plasma. The antibiotic ristocetin, which aggregates human platelets in the presence of von Willebrand factor, nonspecifically precipitates platelet membrane factor VIII antigen. Thus normal platelets contain surface-bound as well as internally stored von Willebrand factor, a protein synthesized by endothelial cells which is necessary for normal platelet function in vivo.

MeSH Terms

Absorption Animals Antigens Blood Platelets/analysis,immunology Cell Membrane/immunology,metabolism Cytoplasmic Granules/immunology Electrophoresis, Polyacrylamide Gel Factor VIII/immunology Humans Immunoelectrophoresis Peptides/analysis Precipitin Tests Proteins/metabolism Rabbits/immunology Ristocetin/metabolism Sodium Dodecyl Sulfate

Chemicals

Antigens Peptides Proteins Ristocetin Sodium Dodecyl Sulfate Factor VIII

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Nachman R L

Jaffe E A

References (26)

26 references, click to expand

Studies on human platelet protease activity.
J Clin Invest. 1969 Nov;47(11):2530-40 PMID: 4237782
Studies on human platelet granules and membranes.
J Clin Invest. 1966 Jan;45(1):14-28 PMID: 4285211
Quantitative assay of a plasma factor deficient in von Willebrand's disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content.
J Clin Invest. 1973 Nov;52(11):2708-16 PMID: 4542944
Decreased adhesion of platelets to subendothelium in von Willebrand's disease.
J Lab Clin Med. 1974 Feb;83(2):296-300 PMID: 4544024
Factor-VIII-related antigen in platelets.
Thromb Res. 1974 May;4(5):617-24 PMID: 4545781
Aggregation of human platelets by bovine or human factor VIII: role of carbohydrate side chains.
Nature. 1973 Jul 20;244(5412):167-8 PMID: 4583498
Synthesis of antihemophilic factor antigen by cultured human endothelial cells.
J Clin Invest. 1973 Nov;52(11):2757-64 PMID: 4583980
Dissociation between human platelet agglomerating activity and factor VIII procoagulant activity of bovine plasma preparations by chemical treatment. I. Effect of neuraminidase.
Pathol Biol (Paris). 1973 Nov;21:Suppl:60-2 PMID: 4587019
Factor VIII antigen in the vessel walls in von Willebrand's disease and haemophilia A.
Scand J Haematol. 1974;13(1):33-8 PMID: 4606548
Immunologic studies of antihemophilic factor (AHF, factor VIII). IV. Radioimmunoassay of AHF antigen.
J Lab Clin Med. 1972 Dec;80(6):822-33 PMID: 4634503
Iodination of the human platelet membrane. Studies of the major surface glycoprotein.
J Biol Chem. 1973 Apr 25;248(8):2928-36 PMID: 4697403
Isolation and characterization of human Factor VIII (antihemophilic factor).
J Biol Chem. 1973 Jun 10;248(11):3946-55 PMID: 4708094
Studies on the mode of action of cholera toxin. Effects on solubilized adenylate cyclase.
J Clin Invest. 1974 Apr;53(4):1202-5 PMID: 4815085
Molecular analysis of the membrane attack mechanism of complement.
J Exp Med. 1972 Mar 1;135(3):549-66 PMID: 5058233
Ristocetin--a new tool in the investigation of platelet aggregation.
Thromb Diath Haemorrh. 1971 Oct 31;26(2):362-9 PMID: 5316292
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.
J Biol Chem. 1970 Jun;245(12):3059-65 PMID: 5432796
Ultrastructural basis of experimental thrombocytopenic purpura.
Arch Pathol. 1970 Sep;90(3):197-205 PMID: 5465553
Immunologic differentiation of classic hemophilia (factor 8 deficiency) and von Willebrand's dissase, with observations on combined deficiencies of antihemophilic factor and proaccelerin (factor V) and on an acquired circulating anticoagulant against antihemophilic factor.
J Clin Invest. 1971 Jan;50(1):244-54 PMID: 5543879
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Immunologic studies of proteins associated with subcellular fractions of normal human platelets.
J Lab Clin Med. 1967 Apr;69(4):651-8 PMID: 6020701
Studies on the nature of the circulating anticoagulant directed against antihemophilic factor: with notes on an assay for anthemophilic factor.
Blood. 1962 Aug;20:137-49 PMID: 13872719
Platelet thrombosthenin: subcellular localization and function.
J Clin Invest. 1967 Aug;46(8):1380-9 PMID: 16695926
Synthesis of von Willebrand factor by cultured human endothelial cells.
Proc Natl Acad Sci U S A. 1974 May;71(5):1906-9 PMID: 4209883
The cold-insoluble globulin of plasma and its relationship to factor VIII.
J Lab Clin Med. 1974 Aug;84(2):258-63 PMID: 4135593
Von Willebrand's disease--diagnostic criteria.
Blood. 1968 Oct;32(4):668-79 PMID: 4176828
Defective ristocetin-induced platelet aggregation in von Willebrand's disease and its correction by factor VIII.
J Clin Invest. 1973 Nov;52(11):2697-707 PMID: 4201262

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.141.5.1101

Published

1975-05-01

Pages

1101-13

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2189790

Subset

External Links

PubMed source DOI: 10.1084/jem.141.5.1101 PMC Full Text View journal details

Analysis Services

Subcellular platelet factor VIII antigen and von Willebrand factor.

Contact

WeChat Official Account