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PMID: 11739797 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Actin filaments and myosin I alpha cooperate with microtubules for the movement of lysosomes.

Molecular biology of the cell ·Vol. 12 ·No. 12 ·2001-12-00 ·Pages 4013-29

Cordonnier MN, Dauzonne D, Louvard D, Coudrier E

Abstract

An earlier report suggested that actin and myosin I alpha (MMIalpha), a myosin associated with endosomes and lysosomes, were involved in the delivery of internalized molecules to lysosomes. To determine whether actin and MMIalpha were involved in the movement of lysosomes, we analyzed by time-lapse video microscopy the dynamic of lysosomes in living mouse hepatoma cells (BWTG3 cells), producing green fluorescent protein actin or a nonfunctional domain of MMIalpha. In GFP-actin cells, lysosomes displayed a combination of rapid long-range directional movements dependent on microtubules, short random movements, and pauses, sometimes on actin filaments. We showed that the inhibition of the dynamics of actin filaments by cytochalasin D increased pauses of lysosomes on actin structures, while depolymerization of actin filaments using latrunculin A increased the mobility of lysosomes but impaired the directionality of their long-range movements. The production of a nonfunctional domain of MMIalpha impaired the intracellular distribution of lysosomes and the directionality of their long-range movements. Altogether, our observations indicate for the first time that both actin filaments and MMIalpha contribute to the movement of lysosomes in cooperation with microtubules and their associated molecular motors.

MeSH Terms
Actin Cytoskeleton/drug effects,metabolism Animals Biological Transport/drug effects Cytochalasin D/pharmacology Green Fluorescent Proteins Luminescent Proteins/metabolism Lysosomes/drug effects,metabolism Mice Microscopy, Video Microtubules/drug effects,metabolism Myosin Type I/metabolism Nocodazole/pharmacology Pepstatins/pharmacology Time Factors Tumor Cells, Cultured
Chemicals
Luminescent Proteins Pepstatins Green Fluorescent Proteins Cytochalasin D Myosin Type I Nocodazole pepstatin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cordonnier M N
Morphogenèse et Signalisation Cellulaires, Unité Mixte de Recherche 144, Institut Curie, France.
Dauzonne D
Louvard D
Coudrier E
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.12.12.4013
Published
2001-12-00
Pages
4013-29
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC60772
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.12.12.4013 PMC Full Text View journal details
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