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PMID: 11744247 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hippocampal mossy fiber activity evokes Ca2+ release in CA3 pyramidal neurons via a metabotropic glutamate receptor pathway.

Neuroscience ·Vol. 107 ·No. 1 ·2001-00-00 ·Pages 59-69

Kapur A, Yeckel M, Johnston D

Abstract

Mossy fiber activity can evoke Ca2+ release from internal stores in CA3 neurons, but the physiological conditions under which this occurs and the mechanisms underlying the release are not understood. Using rat hippocampal slices we report here that short trains of mossy fiber stimulation activate group I metabotropic glutamate receptors (mGluRs) on CA3 pyramidal neurons and elicit waves of Ca2+ release from inositol 1,4,5-trisphosphate (IP3) sensitive internal stores that propagate from stratum lucidum to the soma and in some cases distally out the dendrites. Activation of mGluR1,5 receptors by an agonist trans-azetidine-2,4-dicarboxylic acid (tADA) applied to stratum lucidum was also sufficient to induce waves of Ca2+ release. This release was blocked by internal heparin, but not by dantrolene, suggesting the involvement of IP3 rather than ryanodine receptors in not only the initial release but also in the maintenance of the propagating waves. Release could be facilitated by Ca2+ influx through voltage-gated Ca2+ channels, which is consistent with the known Ca2+ sensitivity of IP3 receptors.These results provide insight into the mechanisms and conditions of Ca2+ release in CA3 neurons and demonstrate the powerful influence mossy fiber input can have on these neurons.

MeSH Terms
Action Potentials/drug effects,physiology Animals Calcium/metabolism Calcium Channels/drug effects,metabolism Calcium Signaling/drug effects,physiology Dantrolene/pharmacology Dendrites/drug effects,metabolism,ultrastructure Electric Stimulation Excitatory Amino Acid Agonists/pharmacology Excitatory Amino Acid Antagonists/pharmacology Heparin/pharmacology Inositol 1,4,5-Trisphosphate Receptors Intracellular Fluid/drug effects,metabolism Mossy Fibers, Hippocampal/drug effects,metabolism,ultrastructure Muscle Relaxants, Central/pharmacology Narcotic Antagonists/pharmacology Neuronal Plasticity/drug effects,physiology Pyramidal Cells/drug effects,physiology Rats Rats, Sprague-Dawley Reaction Time/drug effects,physiology Receptors, Cytoplasmic and Nuclear/antagonists & inhibitors,metabolism Receptors, Metabotropic Glutamate/drug effects,metabolism Ryanodine Receptor Calcium Release Channel/drug effects,metabolism Synaptic Transmission/physiology
Chemicals
Calcium Channels Excitatory Amino Acid Agonists Excitatory Amino Acid Antagonists Inositol 1,4,5-Trisphosphate Receptors Muscle Relaxants, Central Narcotic Antagonists Receptors, Cytoplasmic and Nuclear Receptors, Metabotropic Glutamate Ryanodine Receptor Calcium Release Channel Heparin Dantrolene Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kapur A
Division of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Yeckel M
Johnston D
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34 references, click to expand
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Article Info
Journal
Neuroscience
Abbr.
Neuroscience
ISSN
0306-4522
DOI
10.1016/s0306-4522(01)00293-7
Published
2001-00-00
Pages
59-69
Language
English
Region
United States
NLM ID
7605074
PMCID
PMC2867668
Subset
IM
Grants
NINDS NIH HHS · NS37444 · United States
NIMH NIH HHS · F32 MH011390-03 · United States
NIMH NIH HHS · MH48432 · United States
NINDS NIH HHS · P01 NS037444 · United States
NIMH NIH HHS · F32 MH011390 · United States
NIMH NIH HHS · R37 MH044754 · United States
NIMH NIH HHS · R01 MH048432 · United States
NIMH NIH HHS · MH44754 · United States
External Links
PubMed source DOI: 10.1016/s0306-4522(01)00293-7 PMC Full Text View journal details
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