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PMID: 11779797 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The N terminus of Saccharomyces cerevisiae Sst2p plays an RGS-domain-independent, Mpt5p-dependent role in recovery from pheromone arrest.

Genetics ·Vol. 159 ·No. 4 ·2001-12-00 ·Pages 1559-71

Xu BE, Skowronek KR, Kurjan J

Abstract

The Saccharomyces cerevisiae RGS protein Sst2p is involved in desensitization to pheromone and acts as a GTPase-activating protein for the Galpha subunit Gpa1p. Other results indicate that Sst2p acts through Mpt5p and that this action occurs downstream of Fus3p and through Cln3p/Cdc28p. Our results indicate that the interaction of Sst2p with Mpt5p requires the N-terminal MPI (Mpt5p-interacting) domain of Sst2p and is independent of the C-terminal RGS domain. Overexpression of the MPI domain results in an Mpt5p-dependent increase in recovery from pheromone arrest. Overexpression of either intact Sst2p or the MPI domain leads to partial suppression of a gpa1 growth defect, and this suppression is dependent on Mpt5p, indicating that MPI function occurs downstream of Gpa1p and through Mpt5p. Combination of an mpt5 mutation with the GPA1(G302S) mutation, which uncouples Gpa1p from Sst2p, results in pheromone supersensitivity similar to the sst2 mutant, and promotion of recovery by overexpression of Sst2p is dependent on both Mpt5p and the Gpa1p interaction. These results indicate that Sst2p is a bifunctional protein and that the MPI domain acts through Mpt5p independently of the RGS domain. RGS family members from other fungi contain N-terminal domains with sequence similarity to the Sst2p MPI domain, suggesting that MPI function may be conserved.

MeSH Terms
Amino Acid Sequence Cell Cycle Proteins Escherichia coli/metabolism Fungal Proteins/chemistry,metabolism GTP-Binding Protein alpha Subunits GTP-Binding Protein alpha Subunits, Gq-G11 GTPase-Activating Proteins/metabolism Genes, Fungal Heterotrimeric GTP-Binding Proteins/genetics Mitogen-Activated Protein Kinases/metabolism Models, Biological Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Mutation Phenotype Pheromones/pharmacology Plasmids/metabolism Protein Structure, Tertiary RGS Proteins/chemistry RNA-Binding Proteins Repressor Proteins Saccharomyces cerevisiae/chemistry Saccharomyces cerevisiae Proteins/genetics,physiology Sequence Homology, Amino Acid Two-Hybrid System Techniques
Chemicals
Cell Cycle Proteins Fungal Proteins GTP-Binding Protein alpha Subunits GTPase-Activating Proteins MPT5 protein, S cerevisiae Pheromones RGS Proteins RNA-Binding Proteins Repressor Proteins SST2 protein, S cerevisiae Saccharomyces cerevisiae Proteins FUS3 protein, S cerevisiae Mitogen-Activated Protein Kinases GPA1 protein, S cerevisiae GTP-Binding Protein alpha Subunits, Gq-G11 Heterotrimeric GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xu B E
Department of Microbiology and Molecular Genetics and the Vermont Cancer Center, University of Vermont, College of Medicine, Burlington, Vermont 05405-0068, USA.
Skowronek K R
Kurjan J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/159.4.1559
Published
2001-12-00
Pages
1559-71
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461895
Subset
IM
Grants
NIGMS NIH HHS · GM-40585 · United States
External Links
PubMed source DOI: 10.1093/genetics/159.4.1559 PMC Full Text View journal details
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