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PMID: 11779838 Published · ppublish English Letter Research Support, Non-U.S. Gov't

Analysis of the 5S RNA pool in Arabidopsis thaliana: RNAs are heterogeneous and only two of the genomic 5S loci produce mature 5S RNA.

Genome research ·Vol. 12 ·No. 1 ·2002-01-00 ·Pages 132-44

Cloix C, Tutois S, Yukawa Y, Mathieu O, Cuvillier C, Espagnol MC, Picard G, Tourmente S

Abstract

One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5'- and 3'-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.

MeSH Terms
Arabidopsis/genetics,metabolism Base Sequence Chromosome Mapping DNA Primers/genetics DNA, Ribosomal/genetics Genetic Markers/genetics Genome, Plant Molecular Sequence Data Polymerase Chain Reaction/methods RNA, Heterogeneous Nuclear/genetics RNA, Plant/genetics RNA, Ribosomal, 5S/biosynthesis,genetics Transcription, Genetic
Chemicals
DNA Primers DNA, Ribosomal Genetic Markers RNA, Heterogeneous Nuclear RNA, Plant RNA, Ribosomal, 5S
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cloix Catherine
U.M.R. 6547 BIOMOVE, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France.
Tutois Sylvie
Yukawa Yasushi
Mathieu Olivier
Cuvillier Claudine
Espagnol Marie-Claude
Picard Georges
Tourmente Sylvette
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.181301
Published
2002-01-00
Pages
132-44
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC155267
Subset
IM
Databases
GENBANK
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PubMed source DOI: 10.1101/gr.181301 PMC Full Text View journal details
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